Document Detail


Time-lapse cleavage rating predicts human embryo viability.
MedLine Citation:
PMID:  22881225     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chronology of three consecutive mitotic events in human pre-implantation embryos was examined by time-lapse imaging. In zygotes producing well-formed and pregnancy-yielding expanded blastocysts, uniform time-patterning of cleavage clusters (c) and interphases (i) was revealed: i2=11+/-1, i3=15+/-1, i4=23+/-1 hr / c2=15+/-5, c3=40+/-10, c4=55+/-15 min. Oppositely, shortened or prolonged durations of one or more cell cycles were strongly predictive of poor implantation and development. Furthermore, trichotomic mitosis was discovered in 17 % of cases - zygotes cleaved into 3 blastomeres and 2-cell embryos into 5-6 cells (instead of normal 2 and 4). During conventional clinical assessment, such embryos are indistinguishable from normal, often considered just-in-course of the next cell cycle. Only detailed time-lapse monitoring paced at 10-minute intervals had proven all these embryos to be absolutely unviable, even in rare cases when they reduced their hypercellularity to normal cell counts via cell-cell fusion. Overall, we demonstrate that time-lapse embryo cleavage rating (ECR) as a standalone diagnostic procedure allows for effective identification of viable early embryos with 90 % specificity, while elimination of good-looking but unviable embryos can be assumed with a specificity of 100 %. Thus, making this non-invasive and contactless approach worth of addition to routine embryo screening in clinical IVF programs.
Authors:
D Hlinka; B Kalatova; I Uhrinova; S Dolinska; J Rutarova; J Rezacova; S Lazarovska; M Dudas
Related Documents :
9887365 - Molecular chaperones in the etiology and therapy of cancer.
7782925 - Role of dietary sphingolipids and inhibitors of sphingolipid metabolism in cancer and o...
19665125 - Actomyosin contractility spatiotemporally regulates actin network dynamics in migrating...
11166215 - Heparan sulfate: decoding a dynamic multifunctional cell regulator.
9509415 - Insulin has a limited effect on the cell cycle progression in 3t3 l1 fibroblasts.
23873995 - Shrinking the hammer: micromechanical approaches to morphogenesis.
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-8
Journal Detail:
Title:  Physiological research / Academia Scientiarum Bohemoslovaca     Volume:  -     ISSN:  1802-9973     ISO Abbreviation:  Physiol Res     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9112413     Medline TA:  Physiol Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Fetal Medicine Program, Department of Cell Biology, Šafárik University, Košice. marek.dudas.sk@gmail.com.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Age-associated changes in Ca(2+)-ATPase and oxidative damage in sarcoplasmic reticulum of rat heart.
Next Document:  Genetic strain-dependent protein metabolism and muscle hypertrophy under chronic isometric training ...