| Time-lapse cleavage rating predicts human embryo viability. | |
| | |
MedLine Citation:
|
PMID: 22881225 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Chronology of three consecutive mitotic events in human pre-implantation embryos was examined by time-lapse imaging. In zygotes producing well-formed and pregnancy-yielding expanded blastocysts, uniform time-patterning of cleavage clusters (c) and interphases (i) was revealed: i2=11+/-1, i3=15+/-1, i4=23+/-1 hr / c2=15+/-5, c3=40+/-10, c4=55+/-15 min. Oppositely, shortened or prolonged durations of one or more cell cycles were strongly predictive of poor implantation and development. Furthermore, trichotomic mitosis was discovered in 17 % of cases - zygotes cleaved into 3 blastomeres and 2-cell embryos into 5-6 cells (instead of normal 2 and 4). During conventional clinical assessment, such embryos are indistinguishable from normal, often considered just-in-course of the next cell cycle. Only detailed time-lapse monitoring paced at 10-minute intervals had proven all these embryos to be absolutely unviable, even in rare cases when they reduced their hypercellularity to normal cell counts via cell-cell fusion. Overall, we demonstrate that time-lapse embryo cleavage rating (ECR) as a standalone diagnostic procedure allows for effective identification of viable early embryos with 90 % specificity, while elimination of good-looking but unviable embryos can be assumed with a specificity of 100 %. Thus, making this non-invasive and contactless approach worth of addition to routine embryo screening in clinical IVF programs. |
| | |
Authors:
|
D Hlinka; B Kalatova; I Uhrinova; S Dolinska; J Rutarova; J Rezacova; S Lazarovska; M Dudas |
Related Documents
:
|
9887365 - Molecular chaperones in the etiology and therapy of cancer. 7782925 - Role of dietary sphingolipids and inhibitors of sphingolipid metabolism in cancer and o... 19665125 - Actomyosin contractility spatiotemporally regulates actin network dynamics in migrating... 11166215 - Heparan sulfate: decoding a dynamic multifunctional cell regulator. 9509415 - Insulin has a limited effect on the cell cycle progression in 3t3 l1 fibroblasts. 1445795 - S phase, an evolutionary chromatin condensation state from g1 to g2, in a breast epithe... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-8-8 |
Journal Detail:
|
Title: Physiological research / Academia Scientiarum Bohemoslovaca Volume: - ISSN: 1802-9973 ISO Abbreviation: Physiol Res Publication Date: 2012 Aug |
Date Detail:
|
Created Date: 2012-8-13 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9112413 Medline TA: Physiol Res Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Fetal Medicine Program, Department of Cell Biology, Šafárik University, Košice. marek.dudas.sk@gmail.com. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Age-associated changes in Ca(2+)-ATPase and oxidative damage in sarcoplasmic reticulum of rat heart.
Next Document: Genetic strain-dependent protein metabolism and muscle hypertrophy under chronic isometric training ...