Document Detail


Time-lapse analysis of laser phase shift for noninvasive discrimination of human normal cells and malignant tumor cells.
MedLine Citation:
PMID:  22738695     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Time-lapse analysis of phase shift using phase-shifting laser microscopy (PLM) revealed that the laser phase shifts of primary normal human prostate epithelial cells (PRECs) and human prostatic carcinoma epithelial cell line (PC-3) cells during the mitotic phase were markedly higher than those in the interphase. The phase shift of PC-3 cells during the interphase was markedly lower than that of PRECs throughout the cell cycle. In conclusion, it was proposed that adherent PC-3 cancer cells could be noninvasively discriminated from normal adherent PRECs by the periodical measurement of phase shift using PLM during culture.
Authors:
Mutsumi Takagi; Yuhei Shibaki
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-26
Journal Detail:
Title:  Journal of bioscience and bioengineering     Volume:  -     ISSN:  1347-4421     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100888800     Medline TA:  J Biosci Bioeng     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.
Affiliation:
Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8 Kita-ku, Sapporo 060-8628, Japan.
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