Document Detail


Time-integrated fluorescence cumulant analysis and its application in living cells.
MedLine Citation:
PMID:  23276537     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Time-integrated fluorescence cumulant analysis (TIFCA) is a data analysis technique for fluorescence fluctuation spectroscopy (FFS) that extracts information from the cumulants of the integrated fluorescence intensity. It is the first exact theory that describes the effect of sampling time on FFS experiment. Rebinning of data to longer sampling times helps to increase the signal/noise ratio of the experimental cumulants of the photon counts. The sampling time dependence of the cumulants encodes both brightness and diffusion information of the sample. TIFCA analysis extracts this information by fitting the cumulants to model functions. Generalization of TIFCA to multicolor FFS experiment is straightforward. Here, we present an overview of the theory, its implementation, as well as the benefits and requirements of TIFCA. The questions of why, when, and how to use TIFCA will be discussed. We give several examples of practical applications of TIFCA, particularly focused on measuring molecular interaction in living cells.
Authors:
Bin Wu; Robert H Singer; Joachim D Mueller
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Methods in enzymology     Volume:  518     ISSN:  1557-7988     ISO Abbreviation:  Meth. Enzymol.     Publication Date:  2013  
Date Detail:
Created Date:  2013-01-01     Completed Date:  2013-06-05     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  0212271     Medline TA:  Methods Enzymol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  99-119     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Line
Cells*
Humans
Protein Binding
Proteins / metabolism
RNA, Messenger / analysis
Spectrometry, Fluorescence / methods*
Grant Support
ID/Acronym/Agency:
GM64589/GM/NIGMS NIH HHS; GM84364/GM/NIGMS NIH HHS; GM86217/GM/NIGMS NIH HHS; R01 GM064589/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Proteins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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