| Time-integrated fluorescence cumulant analysis and its application in living cells. | |
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MedLine Citation:
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PMID: 23276537 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Time-integrated fluorescence cumulant analysis (TIFCA) is a data analysis technique for fluorescence fluctuation spectroscopy (FFS) that extracts information from the cumulants of the integrated fluorescence intensity. It is the first exact theory that describes the effect of sampling time on FFS experiment. Rebinning of data to longer sampling times helps to increase the signal/noise ratio of the experimental cumulants of the photon counts. The sampling time dependence of the cumulants encodes both brightness and diffusion information of the sample. TIFCA analysis extracts this information by fitting the cumulants to model functions. Generalization of TIFCA to multicolor FFS experiment is straightforward. Here, we present an overview of the theory, its implementation, as well as the benefits and requirements of TIFCA. The questions of why, when, and how to use TIFCA will be discussed. We give several examples of practical applications of TIFCA, particularly focused on measuring molecular interaction in living cells. |
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Authors:
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Bin Wu; Robert H Singer; Joachim D Mueller |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in enzymology Volume: 518 ISSN: 1557-7988 ISO Abbreviation: Meth. Enzymol. Publication Date: 2013 |
Date Detail:
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Created Date: 2013-01-01 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0212271 Medline TA: Methods Enzymol Country: United States |
Other Details:
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Languages: eng Pagination: 99-119 Citation Subset: IM |
Copyright Information:
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Copyright © 2013 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA; Gruss-Lipper Biophotonic Center, Albert Einstein College of Medicine, Bronx, New York, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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