Document Detail

Time-dependent regulation of yeast glycolysis upon nitrogen starvation depends on cell history.
MedLine Citation:
PMID:  20232995     Owner:  NLM     Status:  MEDLINE    
In this study, the authors investigated how the glycolytic flux was regulated in time upon nitrogen starvation of cells with different growth histories. We have compared cells grown in glucose-limited chemostat cultures under respiratory conditions (low dilution rate of 0.1/h) to cells grown under respirofermentative conditions (high dilution rate of 0.35/h). The fermentative capacity was lower in cells grown under respiratory conditions than in cells grown under respirofermentative conditions, yet more resilient to prolonged nitrogen starvation. The time profiles revealed that the fermentative capacity even increased in cells grown under respiratory conditions during the first hours of nitrogen starvation. In cells grown under respirofermentative conditions the fermentative capacity decreased from the onset of nitrogen starvation. We have applied time-dependent Regulation Analysis to follow the fermentative capacity during nitrogen starvation. In both experiments, diverse categories of regulation were found. However, in the cells grown under respiratory conditions regulation was predominantly metabolic, whereas in the cells grown under respirofermentative conditions hierarchical regulation was dominant. To study the metabolic regulation, concentrations of intracellular metabolites, including allosteric regulators, were measured. The obtained results can explain some aspects of the metabolic regulation, but not all.
K van Eunen; P Dool; A B Canelas; J Kiewiet; J Bouwman; W M van Gulik; H V Westerhoff; B M Bakker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  IET systems biology     Volume:  4     ISSN:  1751-8849     ISO Abbreviation:  IET Syst Biol     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-17     Completed Date:  2010-05-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101301198     Medline TA:  IET Syst Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  157-68     Citation Subset:  IM    
Vrije Universiteit Amsterdam, Department of Molecular Cell Physiology, Amsterdam, The Netherlands.
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MeSH Terms
Fermentation / physiology
Glycolysis / physiology*
Models, Biological*
Nitrogen / metabolism*
Saccharomyces cerevisiae / chemistry,  metabolism,  physiology*
Systems Biology / methods*
Time Factors
Reg. No./Substance:

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