| Time-dependent effect of chlorhexidine surgical prep. | |
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MedLine Citation:
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PMID: 22000737 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Despite continued advances in preoperative preventive measures and aseptic technique, surgical site infections remain a problem. The purpose of this study was to evaluate the time-dependent effectiveness of chlorhexidine, a common surgical preparation solution, at various concentrations. Agar plates containing a Mueller-Hinton medium were inoculated with Staphylococcus aureus (lux) bacteria. The bacteria are genetically engineered to emit photons, allowing for quantification with a photon-counting camera system. Standardized amounts of aqueous chlorhexidine at three different concentrations (group 1:4%; group 2:2%; group 3:0.4%) were applied to the agar plates and comparisons in bacterial reduction were made. After 2min of contact time, groups 1 and 2 had similar reductions in bacterial load with 30% bacterial load remaining in each group (P=0.512), whereas group 3 had a significantly higher bacterial load (33%) when compared to both groups 1 and 2 (1 vs 3, P<0.0001; 2 vs 3, P=0.0002). The bacterial load in all three groups continued to decrease out to the final time point (1h) with group 1 having the least amount of bacterial load remaining, 9% (P<0.0001) and group 3 with the highest bacterial load remaining, 19% (P<0.0001). This study demonstrates two key results: first, dilution of chlorhexidine correlates directly with its bactericidal activity; second, its effectiveness is directly related to its contact time. Based on the results of this study, the authors recommend using 4% chlorhexidine for surgical site preparation and allowing a minimum of 2min of contact time prior to making the skin incision. |
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Authors:
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D J Stinner; C A Krueger; B D Masini; J C Wenke |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-13 |
Journal Detail:
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Title: The Journal of hospital infection Volume: - ISSN: 1532-2939 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8007166 Medline TA: J Hosp Infect Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Published by Elsevier Ltd. |
Affiliation:
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United States Army Institute of Surgical Research, Department of Regenerative Medicine, Fort Sam Houston, TX, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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