Document Detail


Time dependent changes occurring in rat liver microsomes upon lipid peroxidation.
MedLine Citation:
PMID:  2811614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Steady-state fluorescence anisotropy of diphenylhexatriene and n-(9-anthroyloxy)stearic acids (n = 2,12) in rat liver microsomes showed a marked increase in the early stages of enzymatically or non-enzymatically induced lipid peroxidation. The changes in fluorescence anisotropy occurred in parallel with the formation of thiobarbituric acid-reactive substances (TBA-RS). Parallel to these changes, the fluorescence emitted from peroxidized microsomes increased markedly in the early stages of lipid peroxidation. In contrast to the changes in the fluorescence anisotropy and in the formation of TBA-RS, the fluorescence showed a continuing increase over the three hr period of lipid peroxidation. Glucose-6-phosphatase was inactivated in the early stages of lipid peroxidation, whereas NADH-cytochrome b5 reductase underwent a slow deactivation over three hr. The apparently slow deactivation of the peripheral protein may be explained by the formation of fluorescent substances.
Authors:
F Itoh; Y Minamide; T Horie; S Awazu
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Lipids     Volume:  24     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  1989 Oct 
Date Detail:
Created Date:  1989-12-21     Completed Date:  1989-12-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  905-8     Citation Subset:  IM    
Affiliation:
Department of Biopharmaceutics, Tokyo College of Pharmacy, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fluorescence Polarization
Lipid Peroxidation*
Male
Membrane Lipids / metabolism*
Microsomes, Liver / metabolism*
NADP / metabolism
Rats
Rats, Inbred Strains
Thiobarbiturates
Time Factors
Chemical
Reg. No./Substance:
0/Membrane Lipids; 0/Thiobarbiturates; 504-17-6/thiobarbituric acid; 53-59-8/NADP

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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