Document Detail

Time-dependent changes in smooth muscle cell stiffness and focal adhesion area in response to cyclic equibiaxial stretch.
MedLine Citation:
PMID:  18214679     Owner:  NLM     Status:  MEDLINE    
Observations from diverse studies on cell biomechanics and mechanobiology reveal that altered mechanical stimuli can induce significant changes in cytoskeletal organization, focal adhesion complexes, and overall mechanical properties. To investigate effects of short-term equibiaxial stretching on the transverse stiffness of and remodeling of focal adhesions in vascular smooth muscle cells, we developed a cell-stretching device that can be combined with both atomic force and confocal microscopy. Results demonstrate that cyclic 10%, but not 5%, equibiaxial stretching at 0.25 Hz significantly and rapidly alters both cell stiffness and focal adhesion associated paxillin and vinculin. Moreover, measured changes in stiffness and focal adhesion area from baseline values tend to correlate well over the durations of stretching studied. It is suggested that remodeling of focal adhesions plays a critical role in regulating cell stiffness by recruiting and anchoring actin filaments, and that cells rapidly remodel in an attempt to maintain a homeostatic biomechanical state when perturbed above a threshold value.
S Na; A Trache; J Trzeciakowski; Z Sun; G A Meininger; J D Humphrey
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2008-01-24
Journal Detail:
Title:  Annals of biomedical engineering     Volume:  36     ISSN:  1573-9686     ISO Abbreviation:  Ann Biomed Eng     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-11     Completed Date:  2008-03-03     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  0361512     Medline TA:  Ann Biomed Eng     Country:  United States    
Other Details:
Languages:  eng     Pagination:  369-80     Citation Subset:  IM    
Department of Biomedical Engineering, Texas A&M University, 337 Zachry Engineering Center, 3120 TAMU, College Station, TX, 77843-3120, USA.
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MeSH Terms
Cell Adhesion / physiology*
Cells, Cultured
Computer Simulation
Cytoskeletal Proteins / physiology*
Cytoskeleton / physiology*
Focal Adhesions / physiology*
Mechanotransduction, Cellular / physiology*
Models, Biological
Muscle, Smooth, Vascular / physiology*
Myocytes, Smooth Muscle / physiology*
Stress, Mechanical
Time Factors
Grant Support
Reg. No./Substance:
0/Cytoskeletal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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