| Time dependent appearance of selected apoptotic markers and usefulness of their detection in vitro. | |
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MedLine Citation:
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PMID: 12587780 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Many experiments have demonstrated that some cell lines are resistant to chemically induced apoptosis in vitro, and that apoptosis itself is far from being a homogenous phenomenon. Here we show that 10 microg/ml etoposide elicited only minor changes in Bowes human melanoma cells (temporary decrease in cell viability and proliferation, transient phospatidylserine externalization and caspase-3 activation), which weren't clearly capable to start apoptotic pathway in the entire treated population. On the other hand, potassium chromate at concentration of 150 microg/ml executed cell death bearing some features of apoptosis (cell blebbing, caspase-3 activation and cytoskeletal changes) but lacking or showing weakly others (DNA fragmentation and phospatidylserine externalization). Our results suggest that in detecting apoptosis several fault-proof detection systems are to be used to avoid misleading results and conclusions in each experimental setting. |
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Authors:
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Emil Rudolf; Miroslav Cervinka |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta medica (Hradec Králové) / Universitas Carolina, Facultas Medica Hradec Králové Volume: 45 ISSN: 1211-4286 ISO Abbreviation: Acta Medica (Hradec Kralove) Publication Date: 2002 |
Date Detail:
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Created Date: 2003-02-17 Completed Date: 2003-03-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9705947 Medline TA: Acta Medica (Hradec Kralove) Country: Czech Republic |
Other Details:
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Languages: eng Pagination: 135-44 Citation Subset: IM |
Affiliation:
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Charles University in Prague, Faculty of Medicine in Hradec Králové, Department of Medical Biology and Genetics, Czech Republic. rudolf@lfhk.cuni.cz |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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metabolism Annexin A5 / analysis Apoptosis* / drug effects Biological Markers / analysis* Caspase 3 Caspases / metabolism Cell Division Chromates / pharmacology DNA Fragmentation / drug effects DNA, Neoplasm / analysis, drug effects Etoposide / pharmacology Fluorescein-5-isothiocyanate Fluorescent Dyes Humans Melanoma, Experimental / chemistry, pathology Microscopy, Video Phosphatidylserines / analysis Potassium Compounds / pharmacology Tumor Cells, Cultured / chemistry, pathology |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Annexin A5; 0/Biological Markers; 0/Chromates; 0/DNA, Neoplasm; 0/Fluorescent Dyes; 0/Phosphatidylserines; 0/Potassium Compounds; 3326-32-7/Fluorescein-5-isothiocyanate; 33419-42-0/Etoposide; 7789-00-6/potassium chromate(VI); EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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