Document Detail


Time of day regulates subcellular trafficking, tripartite synaptic localization, and polyadenylation of the astrocytic Fabp7 mRNA.
MedLine Citation:
PMID:  22279223     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The astrocyte brain fatty acid binding protein (Fabp7) has previously been shown to have a coordinated diurnal regulation of mRNA and protein throughout mouse brain, and an age-dependent decline in protein expression within synaptoneurosomal fractions. Mechanisms that control time-of-day changes in expression and trafficking Fabp7 to the perisynaptic process are not known. In this study, we confirmed an enrichment of Fabp7 mRNA and protein in the astrocytic perisynaptic compartment, and observed a diurnal change in the intracellular distribution of Fabp7 mRNA in molecular layers of hippocampus. Northern blotting revealed a coordinated time-of-day-dependent oscillation for the Fabp7 mRNA poly(A) tail throughout murine brain. Cytoplasmic polyadenylation element-binding protein 1 (CPEB1) regulates subcellular trafficking and translation of synaptic plasticity-related mRNAs. Here we show that Fabp7 mRNA coimmunoprecipitated with CPEB1 from primary mouse astrocyte extracts, and its 3'UTR contains phylogenetically conserved cytoplasmic polyadenylation elements (CPEs) capable of regulating translation of reporter mRNAs during Xenopus oocyte maturation. Given that Fabp7 expression is confined to astrocytes and neural progenitors in adult mouse brain, the synchronized cycling pattern of Fabp7 mRNA is a novel discovery among known CPE-regulated transcripts. These results implicate circadian, sleep, and/or metabolic control of CPEB-mediated subcellular trafficking and localized translation of Fabp7 mRNA in the tripartite synapse of mammalian brain.
Authors:
Jason R Gerstner; William M Vanderheyden; Timothy LaVaute; Cara J Westmark; Labib Rouhana; Allan I Pack; Marv Wickens; Charles F Landry
Related Documents :
24876753 - Lncrnas expression signatures of hepatocellular carcinoma revealed by microarray.
23546593 - Expression and significance of pten and mir-92 in hepatocellular carcinoma.
17943183 - Increased expression of wnt2 and sfrp4 in tsk mouse skin: role of wnt signaling in alte...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  32     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2012-01-26     Completed Date:  2012-03-12     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1383-94     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Astrocytes / metabolism*
Base Sequence
Cells, Cultured
Circadian Rhythm / physiology*
Fatty Acid-Binding Proteins / metabolism*
Female
Hippocampus / metabolism,  physiology
Male
Mice
Mice, Inbred C57BL
Molecular Sequence Data
Nerve Tissue Proteins / metabolism*
Polyadenylation / physiology
Protein Transport / physiology
RNA, Messenger / metabolism*
Subcellular Fractions / metabolism,  physiology
Synapses / metabolism*,  physiology
Xenopus
Grant Support
ID/Acronym/Agency:
DA13780/DA/NIDA NIH HHS; R01 GM031892/GM/NIGMS NIH HHS; R01 GM031892-15/GM/NIGMS NIH HHS; R37 GM031892/GM/NIGMS NIH HHS; R37 GM031892-25/GM/NIGMS NIH HHS; T32 HL007713/HL/NHLBI NIH HHS; T32 HL007713-19/HL/NHLBI NIH HHS; T32 HL07713/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Fabp7 protein, mouse; 0/Fatty Acid-Binding Proteins; 0/Nerve Tissue Proteins; 0/RNA, Messenger
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Early exposure to alcohol leads to permanent impairment of dendritic excitability in neocortical pyr...
Next Document:  The Amplitude and Timing of the BOLD Signal Reflects the Relationship between Local Field Potential ...