Document Detail

Time course profile and cell-type-specific production of monokine induced by interferon-gamma in Concanavalin A-induced hepatic injury in mice: comparative study with interferon-inducible protein-10.
MedLine Citation:
PMID:  11761028     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: We have previously shown that interferon-inducible protein-10 (IP-10), a chemokine for activated lymphocytes, was specifically induced in the liver of Concanavalin A (Con A)-treated mice. The aim of this study was to investigate the time course profile and cell-type-specific hepatic production of monokine induced by interferon-gamma (MIG), a chemokine which shares its receptor and most of its activity with IP-10, in Con A-treated mice and to compare them with those of IP-10. METHODS: Hepatic mRNA expression of MIG and IP-10 was studied by means of Northern blot analysis and in situ hybridization in Con A-treated mice. The levels of MIG and IP-10 in the serum and culture supernatants of murine hepatoma-, hepatic sinusoidal endothelial cell-, hepatic stellate cell- and macrophage-derived cell lines were determined by means of specific enzyme-linked immunosorbent assays. RESULTS: The serum level of MIG slowly reached a maximum at 12 h after Con A injection and remained elevated for a long time, whereas that of IP-10 reached a maximum at 3 h and declined quickly, a finding supported by Northern blot analysis. Using in situ hybridization, the mRNA of MIG as well as IP-10 was found to be expressed in hepatocytes and hepatic non-parenchymal cells. Similar to IP-10, MIG was produced by hepatoma-, hepatic sinusoidal endothelial cell-, hepatic stellate cell- and macrophage-derived cell lines in vitro. CONCLUSIONS: Although both MIG and IP-10 were produced by hepatocytes and hepatic non-parenchymal cells in Con A-treated mice, the time course profile of MIG was distinguishable from that of IP-10. The fact that hepatic MIG and IP-10 were produced sequentially in this hepatitis model may suggest that a non-redundant role is played by these two chemokines in the process of hepatic necro-inflammation.
Y Itoh; A Morita; K Nishioji; H Fujii; H Nakamura; T Kirishima; T Toyama; N Yamauchi; Y Nagao; S Narumi; T Okanoue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Scandinavian journal of gastroenterology     Volume:  36     ISSN:  0036-5521     ISO Abbreviation:  Scand. J. Gastroenterol.     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2001-12-11     Completed Date:  2002-02-22     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0060105     Medline TA:  Scand J Gastroenterol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  1344-51     Citation Subset:  IM    
Third Dept. of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
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MeSH Terms
Blotting, Northern
Cell Line
Chemokine CXCL10
Chemokine CXCL9
Chemokines, CXC / metabolism*
Concanavalin A*
Enzyme-Linked Immunosorbent Assay
Hepatitis, Animal / metabolism*
In Situ Hybridization
Intercellular Signaling Peptides and Proteins*
Liver / metabolism*
Mice, Inbred C57BL
Time Factors
Reg. No./Substance:
0/CXCL9 protein, human; 0/Chemokine CXCL10; 0/Chemokine CXCL9; 0/Chemokines, CXC; 0/Intercellular Signaling Peptides and Proteins; 11028-71-0/Concanavalin A; 82115-62-6/Interferon-gamma

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