| Time course of prion seeding activity in cerebrospinal fluid of scrapie-infected hamsters after intratongue and intracerebral inoculations. | |
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MedLine Citation:
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PMID: 22238438 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To assess prospects for early diagnosis of prion disease based on prion seeding activity in cerebrospinal fluid (CSF), we measured the activity over time in scrapie-infected hamsters by real-time quaking-induced conversion (RT-QuIC). After intracerebral inoculation, activity appeared in CSF within 1 day and plateaued weeks before the onset of clinical signs. However, after intratongue inoculation, activity first appeared in CSF with the onset of clinical signs, well after higher-level accumulation of seeding activity in brain. |
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Authors:
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Christina D Orrù; Andrew G Hughson; Brent Race; Gregory J Raymond; Byron Caughey |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Intramural Date: 2012-01-11 |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 50 ISSN: 1098-660X ISO Abbreviation: J. Clin. Microbiol. Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-03-19 Completed Date: 2012-07-10 Revised Date: 2013-02-08 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: United States |
Other Details:
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Languages: eng Pagination: 1464-6 Citation Subset: IM |
Affiliation:
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Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amyloid
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cerebrospinal fluid*,
chemistry Animals Brain / pathology Cricetinae Kinetics Limit of Detection Prions / cerebrospinal fluid*, chemistry Scrapie / cerebrospinal fluid*, diagnosis Tongue / pathology |
| Chemical | |
Reg. No./Substance:
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0/Amyloid; 0/Prions |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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