Document Detail


Time course of prion seeding activity in cerebrospinal fluid of scrapie-infected hamsters after intratongue and intracerebral inoculations.
MedLine Citation:
PMID:  22238438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To assess prospects for early diagnosis of prion disease based on prion seeding activity in cerebrospinal fluid (CSF), we measured the activity over time in scrapie-infected hamsters by real-time quaking-induced conversion (RT-QuIC). After intracerebral inoculation, activity appeared in CSF within 1 day and plateaued weeks before the onset of clinical signs. However, after intratongue inoculation, activity first appeared in CSF with the onset of clinical signs, well after higher-level accumulation of seeding activity in brain.
Authors:
Christina D Orrù; Andrew G Hughson; Brent Race; Gregory J Raymond; Byron Caughey
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural     Date:  2012-01-11
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  50     ISSN:  1098-660X     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-03-19     Completed Date:  2012-07-10     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1464-6     Citation Subset:  IM    
Affiliation:
Laboratory of Persistent Viral Diseases, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
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MeSH Terms
Descriptor/Qualifier:
Amyloid / cerebrospinal fluid*,  chemistry
Animals
Brain / pathology
Cricetinae
Kinetics
Limit of Detection
Prions / cerebrospinal fluid*,  chemistry
Scrapie / cerebrospinal fluid*,  diagnosis
Tongue / pathology
Chemical
Reg. No./Substance:
0/Amyloid; 0/Prions
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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