Document Detail

Time course of lung parenchyma remodeling in pulmonary and extrapulmonary acute lung injury.
MedLine Citation:
PMID:  16109834     Owner:  NLM     Status:  MEDLINE    
The aim of this study is to test the hypothesis that the early changes in lung mechanics and the amount of type III collagen fiber do not predict the evolution of lung parenchyma remodeling in pulmonary and extrapulmonary acute lung injury (ALI). For this purpose, we analyzed the time course of lung parenchyma remodeling in murine models of pulmonary and extrapulmonary ALI with similar degrees of mechanical compromise at the early phase of ALI. Lung histology (light and electron microscopy), the amount of elastic and collagen fibers in the alveolar septa, the expression of matrix metalloproteinase-9, and mechanical parameters (lung-resistive and viscoelastic pressures, and static elastance) were analyzed 24 h, 1, 3, and 8 wk after the induction of lung injury. In control (C) pulmonary (p) and extrapulmonary (exp) groups, saline was intratracheally (it; 0.05 ml) instilled and intraperitoneally (ip; 0.5 ml) injected, respectively. In ALIp and ALIexp groups, mice received Escherichia coli lipopolysaccharide (10 microg it and 125 microg ip, respectively). At 24 h, all mechanical and morphometrical parameters, as well as type III collagen fiber content, increased similarly in ALIp and ALIexp groups. In ALIexp, all mechanical and histological data returned to control values at 1 wk. However, in ALIp, static elastance returned to control values at 3 wk, whereas resistive and viscoelastic pressures, as well as type III collagen fibers and elastin, remained elevated until week 8. ALIp showed higher expression of matrix metalloproteinase-9 than ALIexp. In conclusion, insult in pulmonary epithelium yielded fibroelastogenesis, whereas mice with ALI induced by endothelial lesion developed only fibrosis that was repaired early in the course of lung injury. Furthermore, early functional and morphological changes did not predict lung parenchyma remodeling.
Flavia B Santos; Lilian K S Nagato; Nicolau M Boechem; Elnara M Negri; Alberto Guimarães; Vera L Capelozzi; Debora S Faffe; Walter A Zin; Patricia R M Rocco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-08-18
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  100     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2006 Jan 
Date Detail:
Created Date:  2005-12-16     Completed Date:  2006-02-01     Revised Date:  2013-09-26    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  98-106     Citation Subset:  IM    
Laboratories of Respiration Physiology, Carlos Chagas Filho Institute of Biophysics, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
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MeSH Terms
Adaptation, Physiological
Collagen / metabolism
Elastin / metabolism
Extracellular Matrix / metabolism,  pathology
Lung / pathology*,  physiopathology*
Mice, Inbred BALB C
Recovery of Function / physiology*
Respiratory Distress Syndrome, Adult / pathology*,  physiopathology*
Respiratory Mechanics*
Time Factors
Reg. No./Substance:
9007-34-5/Collagen; 9007-58-3/Elastin

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