Document Detail

Time course of early and late changes in plasma DNA in trauma patients.
MedLine Citation:
PMID:  12881444     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Cell-free DNA concentrations increase in the circulation of patients after trauma and may have prognostic potential, but little is know concerning the temporal changes or clearance of the DNA or its relationships with posttraumatic complications. We investigated temporal changes in plasma DNA concentrations in patients after trauma with use of real-time quantitative PCR. METHODS: Serial plasma samples were taken from two trauma populations. In the first study, samples were collected every 20 min from 25 patients within the first 3 h of trauma. In the second study, samples were collected every day from 36 other trauma patients admitted to the intensive care unit (ICU). RESULTS: In the first study, plasma DNA was increased within 20 min of injury and was significantly higher in patients with severe injury and in patients who went on to develop organ failure. In patients with less severe injuries, plasma DNA concentrations decreased toward reference values within 3 h. In the second study, plasma DNA concentrations were higher in patients who developed multiple organ dysfunction syndrome between the second and fourth days of admission than in patients who did not develop the syndrome. In patients who remained in the ICU with continuing organ dysfunction, plasma DNA remained higher than in healthy controls even at 28 days after injury. Most survivors with multiple organ dysfunction syndrome showed an initial very high peak followed by a prolonged smaller increase. CONCLUSIONS: Plasma DNA concentrations increase early after injury and are higher in patients with severe injuries and in those who develop organ failure. Increased plasma DNA persists for days after injuries, especially in patients with multiple organ dysfunction syndrome.
Nicole Y L Lam; Timothy H Rainer; Lisa Y S Chan; Gavin M Joynt; Y M Dennis Lo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  49     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-25     Completed Date:  2003-08-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1286-91     Citation Subset:  IM    
Accident & Emergency Medicine Academic Unit, Department of Chemical Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, New Territories, Hong Kong SAR.
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MeSH Terms
Biological Markers / blood
DNA / blood*
Globins / analysis,  genetics
Intensive Care Units
Multiple Organ Failure / blood,  diagnosis,  mortality
Polymerase Chain Reaction
Time Factors
Wounds and Injuries / blood,  diagnosis*,  mortality
Reg. No./Substance:
0/Biological Markers; 9004-22-2/Globins; 9007-49-2/DNA

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