| Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique. | |
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MedLine Citation:
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PMID: 12870265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this report, we examined whether corrosion cast method is also applicable for the measurement and estimation of the rat major arteries in which subarachnoid hemorrhage (SAH) is produced. Additionally, we have examined the diameters of the rat major arteries following SAH. A total of 0.3 ml autologous blood was injected into the cisterna magna of male Sprague-Dawley rats for the SAH model. A perfusion of a semi-polymerized casting medium was performed, 10 min, 30 min, 1 h, 4 h, 8 h, 1 day, 2 days, 3 days, 5 days, and 7 days after SAH. The brains were corroded in a 10% NaOH solution. The BA and the other major arteries were then measured using scanning electron microscopy (SEM). Macroscopic observation and hematoxylin-eosin (HE) staining were also performed. Using the corrosion cast method, the biphasic contractile response was observed in the BA; 8.3% and 11.6% contractions were observed 30 min and 1 day after SAH, respectively. In addition, there was almost no smooth muscle or adventitial thickening in the chronic stage. In contrast, the dilative response was observed in the internal carotid artery and middle cerebral artery 10 min after SAH. Macroscopic findings and HE staining revealed that the extensive basal subarachnoid hematoma had almost disappeared by day 2. These results indicate that in this model, the minimal spasm, which occurs one day after SAH, can be explained by the small capacity of the rat subarachnoid space and the rapid cerebrospinal fluid washout around major vessels at the cerebral base. Moreover, the present data also show the compensatory dilatation in the ICA and MCA in the early stage after SAH. |
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Authors:
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Shigeki Ono; Isao Date; Keisuke Onoda; Takashi Ohmoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Neurological research Volume: 25 ISSN: 0161-6412 ISO Abbreviation: Neurol. Res. Publication Date: 2003 Jun |
Date Detail:
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Created Date: 2003-07-21 Completed Date: 2003-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7905298 Medline TA: Neurol Res Country: England |
Other Details:
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Languages: eng Pagination: 383-9 Citation Subset: IM |
Affiliation:
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Department of Neurological Surgery, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan. sono@cc.okayama-u.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anterior Cerebral Artery / pathology Basilar Artery / pathology Carotid Artery, Internal / pathology Cerebral Arteries / pathology* Cerebrovascular Circulation Corrosion Casting / methods* Male Middle Cerebral Artery / pathology Rats Rats, Sprague-Dawley Subarachnoid Hemorrhage / pathology* Time Factors |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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