Document Detail

Time course of ciliary neurotrophic factor mRNA expression is coincident with the presence of protoplasmic astrocytes in traumatized rat striatum.
MedLine Citation:
PMID:  7714923     Owner:  NLM     Status:  MEDLINE    
Adrenal grafting for Parkinson's disease has led to modest functional improvement despite poor graft survival. One explanation is a neurotrophic response within the traumatized striatum. This study was undertaken to investigate the time course of the astrocytic response in vivo and in vitro, and the expression of ciliary neurotrophic factor (CNTF) mRNA following striatal injury. Unilateral stereotaxic biopsy of the rat striatum was performed and gelatin sponge (gel-foam) was immediately placed into the biopsy cavity. Rats were sacrificed on days 1, 3, 5, 7, 14, and 28 post biopsy. Immunohistochemical staining of the traumatized striatum with antibodies to glial fibrillary acidic protein (GFAP) was carried out. The reactive astrocytes which appeared within 7 days after trauma were mostly protoplasmic on the basis of morphology, and maximal on day 7, being 30 times the level in the normal striatum. After day 7, fibrous astrocytes appeared and increased up to day 28, while protoplasmic astrocytes decreased. In addition, immunocytochemical double staining of short term cultured astrocytes from the traumatized striatum with anti-A2B5 and anti-GFAP antibodies revealed that 84% and 90% of astrocytes were type 1 astrocytes on days 3 and 7, respectively; however, by day 28 47% of astrocytes were type 2. Northern blot analysis revealed that CNTF mRNA expression was up-regulated and peaked on day 7, coincident with a predominance of protoplasmic astrocytes in vivo and type 1 astrocytes in vitro, respectively. These findings suggest that the expression of CNTF mRNA is part of the early astrocytic response to trauma, particularly associated with protoplasmic astrocytes in vivo and type 1 astrocytes in vitro.(ABSTRACT TRUNCATED AT 250 WORDS)
H Asada; N Y Ip; L Pan; N Razack; M M Parfitt; R J Plunkett
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of neuroscience research     Volume:  40     ISSN:  0360-4012     ISO Abbreviation:  J. Neurosci. Res.     Publication Date:  1995 Jan 
Date Detail:
Created Date:  1995-05-17     Completed Date:  1995-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600111     Medline TA:  J Neurosci Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  22-30     Citation Subset:  IM    
Department of Neurosurgery, SUNY at Buffalo 14209.
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MeSH Terms
Astrocytes / physiology*
Blotting, Northern
Cell Survival
Ciliary Neurotrophic Factor
Corpus Striatum / physiology*
Nerve Tissue Proteins / genetics*
RNA, Messenger / genetics*
Rats, Sprague-Dawley
Time Factors
Reg. No./Substance:
0/Ciliary Neurotrophic Factor; 0/Nerve Tissue Proteins; 0/RNA, Messenger

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