Document Detail

Time course of changes in angiogenesis-related factors in denervated muscle.
MedLine Citation:
PMID:  16866781     Owner:  NLM     Status:  MEDLINE    
AIM: Denervation leads to capillary regression in skeletal muscle. To gain insight into the regulation of this process, we investigated the time course of changes in capillary supply and gene expression of angiogenesis-related factors during muscle denervation. METHOD: Female mice underwent surgery to transect the sciatic nerve, and then the gastrocnemius muscles were isolated at 12 h, 1, 3, 5, 10, 20, or 30 days after surgery. The capillary supply was assessed by immunohistochemistry using anti-PECAM-1/CD31 antibody. The mRNA levels for angiogenesis-related factors were analysed using a real-time polymerase chain reaction. RESULTS: We found that the capillary-to-fibre ratio began to decrease 10 days after muscle denervation and decreased by 52% after 30 days. The levels of mRNA for vascular endothelial growth factor (VEGF), its receptors [fms-like tyrosine kinase (Flt-1) and a kinase insert domain-containing receptor/fetal liver kinase-1 (KDR/Flk-1)], angiopoietin-1 and angiopoietin-2 of denervated muscle were immediately down-regulated after 12 h and remained lower than control muscle until 30 days after muscle denervation. The levels of mRNA for the VEGF receptor, neuropilin-1, angiopoietin receptor and Tie-2 decreased within 12-24 h, but returned to near those of control muscle after 10-20 days, and again decreased after 30 days. CONCLUSIONS: These findings suggest that denervation-induced capillary regression may be associated with down-regulation of VEGF and angiopoietin signalling.
A Wagatsuma; T Osawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  187     ISSN:  1748-1708     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2006 Aug 
Date Detail:
Created Date:  2006-07-26     Completed Date:  2007-03-29     Revised Date:  2009-02-03    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  503-9     Citation Subset:  IM    
Department of Biochemical Sciences, National Institute of Fitness and Sports, Shiromizu, Kanoya, Kagoshima, Japan.
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MeSH Terms
Angiogenesis Inducing Agents / metabolism*
Angiopoietins / genetics,  metabolism
Capillaries / pathology
Mice, Inbred Strains
Muscle Denervation
Muscle, Skeletal / blood supply,  innervation*,  metabolism
Polymerase Chain Reaction / methods
RNA, Messenger / genetics
Time Factors
Tissue Culture Techniques
Vascular Endothelial Growth Factor A / genetics,  metabolism
Reg. No./Substance:
0/Angiogenesis Inducing Agents; 0/Angiopoietins; 0/RNA, Messenger; 0/Vascular Endothelial Growth Factor A

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