| Time course of angiogenesis in solid pineal autografts. | |
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MedLine Citation:
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PMID: 14727176 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Angiogenesis and reperfusion of blood vessels were analysed qualitatively, at the light- and electron-microscopical levels, in solid pineal autografts placed intracerebrally in adult rats (post-transplantation survival times: 1, 3, 7, 10, 14 and 28 days). Reperfusion of blood vessels was studied in sections from immersion-fixed brains incubated to demonstrate the endogenous peroxidase activity of erythrocytes within the lumen of blood vessels. The possible presence of the blood-brain barrier (BBB) within the grafts was also investigated by injecting native horseradish peroxidase (HRP) intravenously into the rats. Angiogenesis, the morphological and functional properties of blood vessels vascularizing the grafts and the survival of pineal tissue were analysed ultrastructurally following transplantation. Revascularization of pineal autografts placed into the adult host central nervous system occurred very slowly, requiring 7-10 days to establish anastomoses between graft and host blood vessels. During this process, signs of angiogenesis in pineal and cerebral capillaries were evident, suggesting that both contributed to graft revascularization. Morphological and functional studies with HRP revealed that, following transplantation, blood vessels at the graft-host interface or within pineal autografts maintained their morphological and functional properties: they were fenestrated and did not present a BBB to blood-borne peroxidase. Thus, after grafting, the presence or absence of the BBB is graft-determined. Revascularized pineal tissue showed good survival and pinealocytes revealed structural features of active secretory cells. |
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Authors:
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Juan Villegas |
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Publication Detail:
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Type: Journal Article Date: 2004-01-15 |
Journal Detail:
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Title: Cell and tissue research Volume: 315 ISSN: 0302-766X ISO Abbreviation: Cell Tissue Res. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-03-01 Completed Date: 2004-10-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0417625 Medline TA: Cell Tissue Res Country: Germany |
Other Details:
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Languages: eng Pagination: 349-59 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Cell Biology, School of Medicine, University of Cantabria, C/Cardenal Herrera Oria s/n, 39011 Santander, Cantabria, Spain. juan.villegas@unican.es |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood-Brain Barrier / physiology Brain / blood supply*, surgery Brain Tissue Transplantation* Cell Survival Female Horseradish Peroxidase / diagnostic use Neovascularization, Pathologic* Pineal Gland / pathology, secretion, transplantation* Rats Rats, Sprague-Dawley Time Factors |
| Chemical | |
Reg. No./Substance:
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EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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