Document Detail

Time course of Fos and Fras expression in the hypothalamic supraoptic neurons during chronic osmotic stimulation.
MedLine Citation:
PMID:  11376854     Owner:  NLM     Status:  MEDLINE    
The Fos family comprises Fos and several subtypes of Fos-related proteins (Fras) such as FosB, Fra-1, Fra-2, DeltaFosB, and chronic Fras. Changes in the expression of Fos family proteins with time are not well elucidated, particularly during chronic stimulation. In the present experiments, we investigated quantitatively the time course changes in Fos, FosB and Fras immunoreactivity in the magnocellular neurons of the supraoptic nucleus (SON) during acute and chronic osmotic stimulation. A small number of Fos- and FosB-positive neurons were observed in the SON of control rats, while many Fras-positive neurons were seen in control animals. Significant increases in the numbers of Fos-, FosB-, and Fras-positive neurons were observed 2 h after acute osmotic stimulation by intraperitoneal (i.p.) injection of 3% NaCl solution. Although the number of Fos-positive neurons returned to the control level 4 h after i.p. injection, a significant number of FosB- and Fras-positive neurons were still observed 8 h after i.p. injection. During chronic osmotic stimulation by giving 2% NaCl solution for 2 and 5 days, a large number of Fos-positive neurons were observed, but the cessation of chronic osmotic stimulation by normal water drinking immediately decreased the number of Fos-positive neurons to the control level within 2 h. The number of FosB-positive neurons was increased with period of chronic osmotic stimulation, and a significant number were observed 2-8 h after the cessation of the stimulation. The number of Fras-positive neurons was also significantly higher during chronic osmotic stimulation, and this number was significantly high 2-8 h after the cessation of the stimulation. RT-PCR analysis demonstrated the persistent expression of c-fos mRNA in the SON during chronic osmotic stimulation. These results suggest that c-fos mRNA and Fos protein are constitutively elevated during chronic osmotic stimulation and the time course changes in Fos are different from those seen in FosB and Fras.
S Miyata; H Tsujioka; M Itoh; W Matsunaga; H Kuramoto; T Kiyohara
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  90     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-29     Completed Date:  2001-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  39-47     Citation Subset:  IM    
Department of Applied Biology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, 606-8585, Kyoto, Japan.
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MeSH Terms
Bacterial Proteins / biosynthesis*,  genetics
DNA-Binding Proteins / biosynthesis*,  genetics
Fos-Related Antigen-2
Gene Expression Regulation / drug effects*
Genes, fos*
Injections, Intraperitoneal
Nerve Tissue Proteins / biosynthesis*,  genetics
Neurons / metabolism*
Osmotic Pressure
Proto-Oncogene Proteins c-fos / biosynthesis*,  genetics
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Saline Solution, Hypertonic / administration & dosage,  pharmacology*
Supraoptic Nucleus / cytology,  metabolism*
Time Factors
Transcription Factors / biosynthesis*,  genetics
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Fos-Related Antigen-2; 0/Fosb protein, rat; 0/Fosl2 protein, rat; 0/Nerve Tissue Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Saline Solution, Hypertonic; 0/Transcription Factors; 0/fos-related antigen 1

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