| Time after excision and temperature alter ex vivo tissue relaxation time measurements. | |
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MedLine Citation:
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PMID: 7981509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously unreported effects of tissue storage were recently observed in the authors' experimental magnetic resonance (MR) studies. To evaluate the effect of elapsed time after excision and storage temperature on tissue relaxation time measurements, tissue samples from the liver, pancreas, kidney, testis, spleen, and brain were obtained in rats. T1 and T2 were first measured within 5 minutes of excision, and between subsequent measurements, tubes were kept in a water bath at 40 degrees C, at room temperature (28 degrees C), or in an ice bath (4 degrees C). Cellular and organellar integrity was assessed with electron microscopy and correlated with the MR findings. At 40 degrees C (20-MHz spectrometer), the T1 of liver decreased from 280 msec +/- 8 to 212 msec +/- 10 during the first 60 minutes; the T1 of pancreas decreased from 276 msec +/- 3 to 208 msec +/- 2. Other tissues showed less than a 5% decrease in T1. T2 changes were smaller than T1 changes in all tissues. Electron microscopy of pancreatic acinar cells showed postmortem changes in mitochondria evolving over the first 60 minutes after death. Manganese loading experiments implicated mitochondrial manganese stores in the observed enhanced postmortem decrease in T1. This study calls into question reported relaxation time data for liver and pancreas. MR studies of excised tissues must account for time and temperature to prevent systematic experimental errors. |
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Authors:
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Y Baba; M M Lerch; D D Stark; A Tanimoto; B P Kreft; L Zhao; A K Saluja; M Takahashi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of magnetic resonance imaging : JMRI Volume: 4 ISSN: 1053-1807 ISO Abbreviation: J Magn Reson Imaging Publication Date: 1994 Sep-Oct |
Date Detail:
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Created Date: 1995-01-05 Completed Date: 1995-01-05 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9105850 Medline TA: J Magn Reson Imaging Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 647-51 Citation Subset: IM |
Affiliation:
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Department of Radiology, Massachusetts General Hospital, Boston. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / drug effects, pathology, surgery Cold Temperature Kidney / drug effects, pathology, surgery Liver / drug effects, pathology*, surgery* Magnesium / pharmacology Magnetic Resonance Spectroscopy* Male Microscopy, Electron Mitochondria / drug effects, ultrastructure Mitochondria, Liver / drug effects, ultrastructure Muscle, Skeletal / drug effects, pathology, surgery Pancreas / drug effects, pathology*, surgery* Rats Rats, Sprague-Dawley Spleen / drug effects, pathology, surgery Temperature Testis / drug effects, pathology, surgery Time Factors Tissue Preservation / methods* |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA50353-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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7439-95-4/Magnesium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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