Document Detail

Time-lapse analysis of cell death in mammalian and fungal cells.
MedLine Citation:
PMID:  20218896     Owner:  NLM     Status:  MEDLINE    
Time-lapse video microscopy was designed to follow the movement of single cells for an unlimited period of time under physiological conditions. The system is based on two inverted microscopes located in a CO(2) incubator and equipped with charge-coupled device cameras connected to the computer. Frames were recorded every minute and the subsequent video sequence was converted to database form. The system was applied to describe the movements of normal HaCaT cells and Pb-treated cells causing the so-called apoptotic dance during cell death. The apoptotic movement was also followed in high-osmolarity glycerol-type mitogen-activated protein kinase (MAPK) null mutant of Fusarium proliferatum, a filamentous fungus, during osmotic stress. The shortest (20 min) and most vigorous death movements were observed in apoptotic fungal cells subjected to salt stress. The necrotic process at higher Pb concentration (50 microM) took 2-3 h, whereas the apoptotic process at lower Pb concentrations lasted from minutes to days.
Gabor Nagy; Gabor Pinter; Gabor Kohut; Attila L Adam; Gyorgy Trencsenyi; Laszlo Hornok; Gaspar Banfalvi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  DNA and cell biology     Volume:  29     ISSN:  1557-7430     ISO Abbreviation:  DNA Cell Biol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-05-14     Completed Date:  2010-05-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9004522     Medline TA:  DNA Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  249-59     Citation Subset:  IM    
Department of Microbial Biotechnology and Cell Biology, University of Debrecen, Debrecen, Hungary.
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MeSH Terms
Cell Death* / drug effects
Cell Division
Cell Line, Transformed
Cell Wall
Chromatin / metabolism
Fusarium / cytology,  physiology
Lead / pharmacology
Microscopy, Video / methods
Stress, Physiological
Yeasts / cytology,  physiology
Reg. No./Substance:
0/Chromatin; 7439-92-1/Lead

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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