Document Detail

Time-kill assay results for a linalool-hinokitiol-based eyelid cleanser for lid hygiene.
MedLine Citation:
PMID:  20308878     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Time-kill testing was performed for a linalool-hinokitiol-based eyelid cleanser against Pseudomonas aeruginosa, Moraxella catarrhalis, Escherichia coli, Serratia marcescens, Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), Staphylococcus epidermidis, and Pityrosporum ovale to evaluate its efficacy. METHODS: An inoculum of 1 mL of each test organism culture was added to 49 mL of test cleanser. At 30 seconds and 1 minute, 1 mL of sample was removed from each test mixture and added to 9 mL of neutralizer. Five milliliters of aliquots were incubated for 48 hours and counted for colony-forming units. Ten percent povidone-iodine was used as a control. RESULTS: At 30 seconds, the linalool-hinokitiol-based eyelid cleanser produced a 99.9999% reduction in P. aeruginosa, a 99.999% reduction in E. coli, a 99.99% reduction in M. catarrhalis and S. marcescens, a 99.9% reduction in MRSA, a 99.2% reduction in S. epidermidis, and a 97.5% reduction in S. aureus. At 1 minute, this cleanser achieved a 95.8% reduction in P. ovale. At 30 seconds, povidone-iodine produced a 99.9999% reduction in P. aeruginosa and M. catarrhalis, a 99.999% reduction in S. marcescens and a 94.9% reduction in S. epidermidis. At 1 minute, 10% povidone-iodine produced a 93.8% reduction in E coli but failed to produce a 1-log reduction in S. aureus or MRSA. CONCLUSIONS: This linalool-hinokitiol-based eyelid cleanser may be helpful in the outpatient setting for patients with presurgical, blepharitis, punctal plug and dry eye, and in the inpatient setting for patients who are allergic to iodine, or as an adjunct to povidone-iodine.
Jeffrey P Gilbard; Yanick Douyon; Robert B Huson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cornea     Volume:  29     ISSN:  1536-4798     ISO Abbreviation:  Cornea     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-26     Completed Date:  2010-06-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8216186     Medline TA:  Cornea     Country:  United States    
Other Details:
Languages:  eng     Pagination:  559-63     Citation Subset:  IM    
Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
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MeSH Terms
Anti-Infective Agents, Local / pharmacology*
Bacteria / drug effects*
Colony Count, Microbial
Disinfectants / pharmacology*
Drug Therapy, Combination
Eyelids / microbiology*
Fungi / drug effects*
Microbial Sensitivity Tests
Monoterpenes / pharmacology*
Tropolone / analogs & derivatives*,  pharmacology
Reg. No./Substance:
0/Anti-Infective Agents, Local; 0/Disinfectants; 0/Monoterpenes; 499-44-5/beta-thujaplicin; 533-75-5/Tropolone; 78-70-6/linalool

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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