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Time Dependence of Cellular Chemical Changes Induced in Prostate PC-3 Cancer Cells by Two Structurally Related Cardenolides Monitored by Fourier Transform Infrared (FT-IR) Spectroscopy.
MedLine Citation:
PMID:  21639979     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Human PC-3 prostate cancer cells were incubated in the presence of two cardenolides, i.e., ouabain and 19-hydroxy-2''-oxovoruscharin. Their effects were monitored by infrared spectroscopy of the cells after different exposure times to the cardenolides. Analysis of changes in absorbance intensities indicated that, for both compounds, the absorbance at one wavenumber with a minor contribution of a second wavenumber is sufficient to build a linear model accurate enough to assign more than 97% of the spectra to their correct time slot. Student t-tests and twodimensional correlation analysis (2D-COS) indicated that both drugs have very similar effects on PC-3 cells. However, asynchronous 2D maps revealed significant differences and allowed the sequence of the spectral changes to be determined: 1395 → 1695 cm(-1) for ouabain, and 1400 → 1655 → 1100 → 1250 → 1020 cm(-1) for 19-hydroxy-2''-oxovoruscharin. 2D correlation map subtraction allowed the identification of very specific differences in the impact of both compounds on PC-3 cells, in particular the ability of 19-hydroxy-2''-oxovoruscharin to affect nucleic acid of PC-3 cells.
Authors:
Régis Gasper; Tatjana Mijatovic; Robert Kiss; Erik Goormaghtigh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Applied spectroscopy     Volume:  65     ISSN:  1943-3530     ISO Abbreviation:  Appl Spectrosc     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372406     Medline TA:  Appl Spectrosc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  584-94     Citation Subset:  IM    
Affiliation:
Laboratory for the Structure and Function of Biological Membranes, Center for Structural Biology and Bioinformatics, Université Libre de Bruxelles (ULB), Brusseles, Belgium.
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