Document Detail


Tightly regulated and heritable division control in single bacterial cells.
MedLine Citation:
PMID:  18469083     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The robust surface adherence property of the aquatic bacterium Caulobacter crescentus permits visualization of single cells in a linear microfluidic culture chamber over an extended number of generations. The division rate of Caulobacter in this continuous-flow culture environment is substantially faster than in other culture apparati and is independent of flow velocity. Analysis of the growth and division of single isogenic cells reveals that the cell cycle control network of this bacterium generates an oscillatory output with a coefficient of variation lower than that of all other bacterial species measured to date. DivJ, a regulator of polar cell development, is necessary for maintaining low variance in interdivision timing, as transposon disruption of divJ significantly increases the coefficient of variation of both interdivision time and the rate of cell elongation. Moreover, interdivision time and cell division arrest are significantly correlated between mother and daughter cells, providing evidence for epigenetic inheritance of cell division behavior in Caulobacter. The single-cell growth/division results reported here suggest that future predictive models of Caulobacter cell cycle regulation should include parameters describing the variance and inheritance properties of this system.
Authors:
Dan Siegal-Gaskins; Sean Crosson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-05-09
Journal Detail:
Title:  Biophysical journal     Volume:  95     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-28     Completed Date:  2008-08-28     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2063-72     Citation Subset:  IM    
Affiliation:
Department of Physics, and The Committee on Microbiology, University of Chicago, Chicago, Illinois 60637, USA.
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MeSH Terms
Descriptor/Qualifier:
Caulobacter crescentus / cytology*,  physiology*
Cell Culture Techniques / methods*
Cell Division / physiology*
Cell Separation / methods*
Feedback
Microfluidics / methods*
Comments/Corrections
Erratum In:
Biophys J. 2009 Nov 4;97(9):2652

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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