Document Detail


Tightly bound binary toxin in the cell wall of Bacillus sphaericus.
MedLine Citation:
PMID:  12089007     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have shown that urea-extracted cell wall of entomopathogenic Bacillus sphaericus 2297 and some other strains is a potent larvicide against Culex pipiens mosquitoes, with 50% lethal concentrations comparable to that of the well-known B. sphaericus binary toxin, with which it acts synergistically. The wall toxicity develops in B. sphaericus 2297 cultures during the late logarithmic stage, earlier than the appearance of the binary toxin crystal. It disappears with sporulation when the binary toxin activity reaches its peak. Disruption of the gene for the 42-kDa protein (P42) of the binary toxin abolishes both cell wall toxicity and crystal formation. However, the cell wall of B. sphaericus 2297, lacking P42, kills C. pipiens larvae when mixed with Escherichia coli cells expressing P42. Thus, the cell wall toxicity in strongly toxic B. sphaericus strains must be attributed to the presence in the cell wall of tightly bound 51-kDa (P51) and P42 binary toxin proteins. The synergism between binary toxin crystals and urea-treated cell wall preparations reflects suboptimal distribution of binary toxin subunits in both compartments. Binary toxin crystal is slightly deficient in P51, while cell wall is lacking in P42.
Authors:
Daniela Klein; Igor Uspensky; Sergei Braun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  68     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-06-28     Completed Date:  2002-07-29     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3300-7     Citation Subset:  IM    
Affiliation:
Department of Biological Chemistry, A. Silberman Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacillus / metabolism*
Bacterial Toxins / metabolism*,  pharmacology
Cell Wall / metabolism*
Culicidae / drug effects
Drug Synergism
Egg Proteins / metabolism
Enzymes / metabolism
Gene Expression
Hot Temperature
Larva / drug effects
Membrane Glycoproteins / metabolism
Receptors, Cell Surface*
Urea / chemistry
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Egg Proteins; 0/Enzymes; 0/Membrane Glycoproteins; 0/Receptors, Cell Surface; 0/binB protein, Bacillus sphaericus; 0/zona pellucida glycoproteins; 57-13-6/Urea
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