Document Detail


The tick salivary protein sialostatin L inhibits the Th9-derived production of the asthma-promoting cytokine IL-9 and is effective in the prevention of experimental asthma.
MedLine Citation:
PMID:  22327077     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Ticks developed a multitude of different immune evasion strategies to obtain a blood meal. Sialostatin L is an immunosuppressive cysteine protease inhibitor present in the saliva of the hard tick Ixodes scapularis. In this study, we demonstrate that sialostatin L strongly inhibits the production of IL-9 by Th9 cells. Because we could show recently that Th9-derived IL-9 is essentially involved in the induction of asthma symptoms, sialostatin L was used for the treatment of experimental asthma. Application of sialostatin L in a model of experimental asthma almost completely abrogated airway hyperresponsiveness and eosinophilia. Our data suggest that sialostatin L can prevent experimental asthma, most likely by inhibiting the IL-9 production of Th9 cells. Thus, alternative to IL-9 neutralization sialostatin L provides the basis for the development of innovative therapeutic strategies to treat asthma.
Authors:
Helena Horka; Valérie Staudt; Matthias Klein; Christian Taube; Sebastian Reuter; Nina Dehzad; John F Andersen; Jan Kopecky; Hansjörg Schild; Michalis Kotsyfakis; Markus Hoffmann; Bastian Gerlitzki; Michael Stassen; Tobias Bopp; Edgar Schmitt
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-02-10
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  188     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-05     Completed Date:  2012-04-19     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2669-76     Citation Subset:  AIM; IM    
Affiliation:
Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic and Faculty of Science, University of South Bohemia, 37005 České Budějovice, Czech Republic.
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MeSH Terms
Descriptor/Qualifier:
Animals
Asthma / immunology*,  metabolism,  prevention & control
Cell Separation
Cystatins / immunology*,  pharmacology
Cytokines / immunology,  metabolism
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Flow Cytometry
Interleukin-9 / biosynthesis,  immunology*
Ixodidae / immunology*
Male
Mice
Mice, Inbred BALB C
Mice, Knockout
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocyte Subsets / immunology*,  metabolism
Grant Support
ID/Acronym/Agency:
Z01 AI001012-01/AI/NIAID NIH HHS; Z01 AI001012-02/AI/NIAID NIH HHS; ZIA AI001012-03/AI/NIAID NIH HHS; ZIA AI001012-04/AI/NIAID NIH HHS; ZIA AI001012-05/AI/NIAID NIH HHS; ZIA AI001012-06/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Cystatins; 0/Cytokines; 0/Interleukin-9; 0/sialostatin L, Ixodes scapularis
Comments/Corrections

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