Document Detail

Thyrotropin-releasing hormone-stimulated thyrotropin expression involves islet-brain-1/c-Jun N-terminal kinase interacting protein-1.
MedLine Citation:
PMID:  15345675     Owner:  NLM     Status:  MEDLINE    
Islet-brain-1 (IB1)/c-Jun N-terminal kinase interacting protein 1 (JIP-1) is a scaffold protein that is expressed at high levels in neurons and the endocrine pancreas. IB1/JIP-1 interacts with the c-Jun N-terminal kinase and mediates the specific physiological stimuli (such as cytokines). However, the potential role of the protein in the pituitary has not been evaluated. Herein, we examined expression of the gene encoding IB1/JIP-1 and its translated product in the anterior pituitary gland and a pituitary cell line, GH3. We then examined the potential role of IB1/JIP-1 in controlling TSH-beta gene expression. Exposure of GH3 cells to TRH stimulated the expression of IB1/JIP-1 protein levels, mRNA, and transcription of the promoter. The increase of IB1/JIP-1 content by transient transfection study of a vector encoding IB1/JIP-1 or by the stimulation of TRH stimulates TSH-beta promoter activity. This effect is not found in the presence of a mutated nonfunctional (IB1S59N) IB1/JIP-1 protein. Together, these facts point to a central role of the IB1/JIP-1 protein in the control of TRH-mediated TSH-beta stimulation.
Hiroshi Abe; Koji Murao; Hitomi Imachi; Wen M Cao; Xiao Yu; Kazuya Yoshida; Norman C W Wong; Margaret A Shupnik; Jacques-Antoine Haefliger; Gérard Waeber; Toshihiko Ishida
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Publication Detail:
Type:  Journal Article     Date:  2004-09-02
Journal Detail:
Title:  Endocrinology     Volume:  145     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-16     Completed Date:  2004-12-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5623-8     Citation Subset:  AIM; IM    
First Department of Internal Medicine, Faculty of Medicine, Kagawa University, Kagawa 761-0793, Japan.
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MeSH Terms
Adaptor Proteins, Signal Transducing / genetics*,  metabolism*
Cell Line, Transformed
Gene Expression Regulation / drug effects,  physiology
Pituitary Gland, Anterior / cytology,  physiology*
Thyrotropin, beta Subunit / genetics*
Thyrotropin-Releasing Hormone / pharmacology*
Transcription, Genetic / drug effects,  physiology
Reg. No./Substance:
0/Adaptor Proteins, Signal Transducing; 0/Mapk8ip protein, rat; 0/Thyrotropin, beta Subunit; 24305-27-9/Thyrotropin-Releasing Hormone

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