| Thyrotropin (TSH)-induced receptor internalization in nonthyroidal cells transfected with a human TSH-receptor complementary deoxyribonucleic acid. | |
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MedLine Citation:
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PMID: 8156904 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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TSH-induced desensitization was studied in nonthyroidal cells expressing functionally active TSH receptors (TSHR). Chinese hamster ovary (CHO) cells and mouse NIH 3T3 cells were stably transfected with a human TSHR cDNA. Stimulation of the CHO-TSHR and NIH-TSHR cells with 10 mU/ml TSH resulted in a decreased sensitivity to a second TSH stimulation only in the NIH-TSHR cells. A decrease in TSH-induced cAMP was present within 1 h and coincided with a decreased binding of [125I]TSH. The half-maximal effect was observed after a 3- to 4-h stimulation with TSH, and exposure of cells to TSH for 20 h led to a 70-80% inhibition of cAMP formation. After withdrawal of TSH, cells regained full responsiveness to TSH after 6 h. Moreover, the desensitization effect observed in NIH-TSHR cells was not mimicked by forskolin and, therefore, was not mediated by cAMP. Stimulation of the CHO-TSHR cells with TSH did not result in a desensitization toward a second TSH stimulation, nor did it reduce the binding of [125I]TSH. This difference between the two cell lines might be explained by a higher turnover rate of receptors in the CHO cells. Indeed, incubation of cells with [125I]TSH showed a more efficient internalization of ligand in the CHO-TSHR cells compared to the NIH-TSHR cells. In summary, the homologous desensitization observed in TSHR-transfected NIH 3T3 cells appears to be the result of ligand-induced receptor down-regulation. |
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Authors:
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N E Heldin; B Gustavsson; A Hermansson; B Westermark |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Endocrinology Volume: 134 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1994 May |
Date Detail:
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Created Date: 1994-05-16 Completed Date: 1994-05-16 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2032-6 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology, University Hospital, Uppsala, Sweden. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Animals CHO Cells Cricetinae Cyclic AMP / metabolism DNA, Complementary / genetics* Endocytosis Genetic Vectors Humans Kinetics Mice Receptors, Thyrotropin / drug effects, genetics, metabolism* Simian virus 40 / genetics Thyroid Neoplasms Thyrotropin / metabolism, pharmacology* Transfection* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA, Complementary; 0/Receptors, Thyrotropin; 60-92-4/Cyclic AMP; 9002-71-5/Thyrotropin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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