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Thyroid tuberculosis - role of PCR in diagnosis of a rare entity.
MedLine Citation:
PMID:  21118313     Owner:  NLM     Status:  In-Data-Review    
N. Gupta, K. Sharma, A. Barwad, M. Sharma, A. Rajwanshi, P. Dutta and A. Sharma Thyroid tuberculosis - role of PCR in diagnosis of a rare entity Background:  Tuberculosis is a rare cause of granulomatous thyroiditis, whose diagnosis may be difficult with routine cytopathology and staining for acid-fast bacilli (AFB). Study design:  Amongst 7962 cases of various thyroid lesions subjected to fine needle aspiration cytology (FNAC) over a period of 12 years, 34 cases (0.43%) were found to have cytological features of granulomatous inflammation with or without necrosis, which could be due to tuberculosis, granulomatous thyroiditis or other causes of granulomatous inflammation such as sarcoidosis or fungal infections. DNA was extracted from the material available on May-Grünwald-Giemsa-stained smears from the archival material. PCR for Mycobacterium tuberculosis was performed for insertion sequence IS6110. Results:  The age of the patients ranged from 32 to 58 years (median 48 years); 24 were female and 10 male. FNAC from thyroid swellings showed epithelioid granulomas with giant cells and/or necrosis. Although acid-fast bacilli were only seen in smears in two cases, 19/34 (55.9%) showed the presence of 123 bp DNA band under ultraviolet transillumination. Five control cases were negative. Conclusion:  Our study of archival cytological material illustrates the importance of PCR as a potentially useful tool for the detection of M. tuberculosis DNA from FNAC of thyroid lesions, which could provide an alternative for rapid diagnosis of thyroid tuberculosis in AFB-negative cases.
N Gupta; K Sharma; A Barwad; M Sharma; A Rajwanshi; P Dutta; A Sharma
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Publication Detail:
Type:  Journal Article     Date:  2010-11-30
Journal Detail:
Title:  Cytopathology : official journal of the British Society for Clinical Cytology     Volume:  22     ISSN:  1365-2303     ISO Abbreviation:  Cytopathology     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010345     Medline TA:  Cytopathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  392-6     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Departments of Cytopathology and Gynaecological Pathology Medical Microbiology Endocrinology Internal Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
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