| Thyroid-stimulating hormone increases active transport of perchlorate into thyroid cells. | |
| | |
MedLine Citation:
|
PMID: 18303123 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Perchlorate blocks thyroidal iodide transport in a dose-dependent manner. The human sodium/iodide symporter (NIS) has a 30-fold higher affinity for perchlorate than for iodide. However, active transport of perchlorate into thyroid cells has not previously been demonstrated by direct measurement techniques. To demonstrate intracellular perchlorate accumulation, we incubated NIS-expressing FRTL-5 rat thyroid cells in various concentrations of perchlorate, and we used a sensitive ion chromatography tandem mass spectrometry method to measure perchlorate accumulation in the cells. Perchlorate caused a dose-related inhibition of 125-iodide uptake at 1-10 microM. The perchlorate content from cell lysate was analyzed, showing a higher amount of perchlorate in cells that were incubated in medium with higher perchlorate concentration. Thyroid-stimulating hormone increased perchlorate uptake in a dose-related manner, thus supporting the hypothesis that perchlorate is actively transported into thyroid cells. Incubation with nonradiolabeled iodide led to a dose-related reduction of intracellular accumulation of perchlorate. To determine potential toxicity of perchlorate, the cells were incubated in 1 nM to 100 microM perchlorate and cell proliferation was measured. Even the highest concentration of perchlorate (100 microM) did not inhibit cell proliferation after 72 h of incubation. In conclusion, perchlorate is actively transported into thyroid cells and does not inhibit cell proliferation. |
| | |
Authors:
|
Neil Tran; Liza Valentín-Blasini; Benjamin C Blount; Caroline Gibbs McCuistion; Mike S Fenton; Eric Gin; Andrew Salem; Jerome M Hershman |
Related Documents
:
|
2868883 - Somatostatin production by a human medullary thyroid carcinoma cell line. 3676833 - The afferent innervation of the thymus gland in the rat. 1168653 - Effects of cytochalasin b on the aggregation, electrophoretic mobility and surface morp... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-02-26 |
Journal Detail:
|
Title: American journal of physiology. Endocrinology and metabolism Volume: 294 ISSN: 0193-1849 ISO Abbreviation: Am. J. Physiol. Endocrinol. Metab. Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-04-01 Completed Date: 2008-05-22 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100901226 Medline TA: Am J Physiol Endocrinol Metab Country: United States |
Other Details:
|
Languages: eng Pagination: E802-6 Citation Subset: IM |
Affiliation:
|
Endocrine Research Laboratory, Veterans Affairs Medical Center West Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, CA 90073, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Binding, Competitive Biological Transport, Active / drug effects, physiology Cell Division / drug effects Cells, Cultured Dose-Response Relationship, Drug Iodides / pharmacokinetics Perchloric Acid / pharmacokinetics* Rats Spectrometry, Mass, Electrospray Ionization Symporters / metabolism Thyroid Gland / cytology*, drug effects, metabolism Thyrotropin / metabolism*, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Iodides; 0/Symporters; 0/perchlorate; 0/sodium-iodide symporter; 7601-90-3/Perchloric Acid; 9002-71-5/Thyrotropin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: JNK- and IkappaB-dependent pathways regulate MCP-1 but not adiponectin release from artificially hyp...
Next Document: Pharmacokinetics of intravenous levosimendan and its metabolites in subjects with hepatic impairment...