Document Detail

Thyroid-stimulating hormone increases active transport of perchlorate into thyroid cells.
MedLine Citation:
PMID:  18303123     Owner:  NLM     Status:  MEDLINE    
Perchlorate blocks thyroidal iodide transport in a dose-dependent manner. The human sodium/iodide symporter (NIS) has a 30-fold higher affinity for perchlorate than for iodide. However, active transport of perchlorate into thyroid cells has not previously been demonstrated by direct measurement techniques. To demonstrate intracellular perchlorate accumulation, we incubated NIS-expressing FRTL-5 rat thyroid cells in various concentrations of perchlorate, and we used a sensitive ion chromatography tandem mass spectrometry method to measure perchlorate accumulation in the cells. Perchlorate caused a dose-related inhibition of 125-iodide uptake at 1-10 microM. The perchlorate content from cell lysate was analyzed, showing a higher amount of perchlorate in cells that were incubated in medium with higher perchlorate concentration. Thyroid-stimulating hormone increased perchlorate uptake in a dose-related manner, thus supporting the hypothesis that perchlorate is actively transported into thyroid cells. Incubation with nonradiolabeled iodide led to a dose-related reduction of intracellular accumulation of perchlorate. To determine potential toxicity of perchlorate, the cells were incubated in 1 nM to 100 microM perchlorate and cell proliferation was measured. Even the highest concentration of perchlorate (100 microM) did not inhibit cell proliferation after 72 h of incubation. In conclusion, perchlorate is actively transported into thyroid cells and does not inhibit cell proliferation.
Neil Tran; Liza Valentín-Blasini; Benjamin C Blount; Caroline Gibbs McCuistion; Mike S Fenton; Eric Gin; Andrew Salem; Jerome M Hershman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-02-26
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  294     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-01     Completed Date:  2008-05-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E802-6     Citation Subset:  IM    
Endocrine Research Laboratory, Veterans Affairs Medical Center West Los Angeles, University of California Los Angeles School of Medicine, Los Angeles, CA 90073, USA.
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MeSH Terms
Binding, Competitive
Biological Transport, Active / drug effects,  physiology
Cell Division / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Iodides / pharmacokinetics
Perchloric Acid / pharmacokinetics*
Spectrometry, Mass, Electrospray Ionization
Symporters / metabolism
Thyroid Gland / cytology*,  drug effects,  metabolism
Thyrotropin / metabolism*,  pharmacology*
Reg. No./Substance:
0/Iodides; 0/Symporters; 0/perchlorate; 0/sodium-iodide symporter; 7601-90-3/Perchloric Acid; 9002-71-5/Thyrotropin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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