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Thyroid peroxidase forms thionamide-sensitive homodimers: relevance for immunomodulation of thyroid autoimmunity.
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PMID:  19669106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thyroid peroxidase (TPO) is the key enzyme in thyroid hormone production and a universal autoantigen in Graves' and other autoimmune thyroid diseases. We wished to explore the expression of TPO and whether it was affected by thionamide antithyroid drugs. We studied recombinant TPO, stably expressed by a Chinese hamster ovary cell line (CHO-TPO) and transiently expressed TPO-enhanced green fluorescent protein (eGFP) and -FLAG fusion proteins. Immunoblotting of CHO-TPO cell extracts showed high-molecular weight (HMW) TPO isoforms that were resistant to reduction, as well as 110 kDa monomeric TPO. Co-immunoprecipitation and enzyme-linked-immunosorbent assay (ELISA) binding studies of FLAG- and eGFP-tagged TPO demonstrated TPO dimerisation. CHO-TPO cells cultured in methimazole (MMI) for 10 days showed a significant reduction in HMW-TPO isoforms at MMI concentrations of 1 microM and above (p < 0.01), whereas monomeric TPO expression was unchanged. We observed a similar reduction in HMW-TPO in CHO-TPO cells cultured in propylthiouracil (10 microM and above). Binding of Graves' disease patient sera and TPO-Fabs to enzymatically active TPO that was captured onto solid phase was not abrogated by MMI. The cellular localisation of TPO in CHO-TPO cells was unchanged by MMI treatment. Our demonstration of homodimeric TPO and the reduction in HMW-TPO isoforms during thionamide treatment of CHO-TPO cells shows, for the first time, an effect of thionamides on TPO structure. This suggests a structural correlate to the effect of thionamides on TPO enzymatic activity and opens up a novel potential mechanism for thionamide immunomodulation of autoimmune thyroid disease.
Authors:
David O McDonald; Simon H S Pearce
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-08
Journal Detail:
Title:  Journal of molecular medicine (Berlin, Germany)     Volume:  87     ISSN:  1432-1440     ISO Abbreviation:  J. Mol. Med.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-06     Completed Date:  2009-12-04     Revised Date:  2011-07-08    
Medline Journal Info:
Nlm Unique ID:  9504370     Medline TA:  J Mol Med (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  971-80     Citation Subset:  IM    
Affiliation:
Institute of Human Genetics, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antithyroid Agents / metabolism
Autoimmunity / immunology*
CHO Cells
Cricetinae
Cricetulus
Humans
Immunomodulation / immunology*
Iodide Peroxidase / chemistry*,  genetics,  metabolism*
Methimazole / metabolism
Molecular Weight
Propylthiouracil / metabolism
Protein Isoforms / chemistry,  genetics,  metabolism
Protein Multimerization
Protein Structure, Quaternary*
Recombinant Fusion Proteins / chemistry,  genetics,  metabolism
Thiazoles* / metabolism
Thyroid Gland / immunology*
Grant Support
ID/Acronym/Agency:
//Medical Research Council
Chemical
Reg. No./Substance:
0/Antithyroid Agents; 0/Protein Isoforms; 0/Recombinant Fusion Proteins; 0/Thiazoles; 51-52-5/Propylthiouracil; 60-56-0/Methimazole; EC 1.11.1.8/Iodide Peroxidase
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Journal ID (nlm-ta): J Mol Med
ISSN: 0946-2716
ISSN: 1432-1440
Publisher: Springer-Verlag, Berlin/Heidelberg
Article Information
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? The Author(s) 2009
Received Day: 8 Month: 6 Year: 2009
Revision Received Day: 1 Month: 7 Year: 2009
Accepted Day: 14 Month: 7 Year: 2009
Electronic publication date: Day: 8 Month: 8 Year: 2009
Print publication date: Month: 10 Year: 2009
Volume: 87 Issue: 10
First Page: 971 Last Page: 980
ID: 2757584
PubMed Id: 19669106
Publisher Id: 511
DOI: 10.1007/s00109-009-0511-y
issue-copyright-statement: ? Springer-Verlag 2009

Thyroid peroxidase forms thionamide-sensitive homodimers: relevance for immunomodulation of thyroid autoimmunity
David O. McDonaldAff1
Simon H. S. PearceAff1 Address: +44-191-2418674 +44-191-2418666 s.h.s.pearce@ncl.ac.uk
Institute of Human Genetics, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne, NE1 3BZ UK

Introduction

Thyroid peroxidase (TPO) is the commonest autoantigen for mankind, 5% to 18% of individuals in Westernised populations showing circulating anti-TPO antibodies, with an age-related increase in prevalence. Furthermore, anti-TPO antibodies are practically universal in individuals with all clinical forms of autoimmune thyroid disease (AITD) [1]. TPO functions as a cell-surface enzyme and is predicted to have four modular domains [2]: an amino-terminal myeloperoxidase (MPO)-like domain, a complement-control protein (CCP)-like domain (also known as a sushi domain), an epidermal growth factor-like domain, and a short carboxy-terminal membrane-anchoring domain. The anti-TPO immune response in AITDs is dominated by antibodies that recognise specific conformational epitopes, termed immunodominant regions, which appear to be composed of structural residues from both its MPO-like and CCP-like domains [3?6]. The enzymatic activity of TPO is essential for thyroid hormone biosynthesis, and molecular abnormalities causing defective TPO activity lead to profound congenital hypothyroidism and goitre [7].

Thionamide compounds, including propylthiouracil (PTU), methimazole (MMI), and carbimazole, have been used to treat Graves? disease and other forms of hyperthyroidism for more than 60?years [8]. Their antithyroid action is mediated by their ability to act as a preferential substrate for TPO. The first step in thyroid hormone synthesis is iodination of thyroglobulin tyrosyl residues. In the presence of thionamide, TPO iodinates the sulphenyl group of the thionamide drug, instead of the thyroglobulin tyrosyl residue. During treatment of hyperthyroidism, thionamides accumulate in the thyroid follicular cells over time. Their increasing concentration results in reduced thyroid hormone synthesis and eventually leads to a complete blockade of thyroid hormone production [9]. An additional effect of thionamides is that they may result in a lasting remission from hyperthyroid Graves? disease, during which, certain individuals may become almost indefinitely euthyroid, even after thionamide administration is stopped [10, 11]. This durable remission of Graves? disease occurs in 40% to 50% of patients who are treated with thionamides and appears to occur irrespective of which compound has been administered, or the dose used [12]. Optimal remission rates of hyperthyroid Graves? disease occur after 6 to 18?months of thionamide administration and are associated with a reduction in circulating antithyroid antibodies, including those directed against the thyrotropin receptor (TSHR) and TPO epitopes [13, 14]. This latter, ?remission-inducing? property of thionamides in Graves? hyperthyroidism has been referred to as an immunomodulatory action. While the enzymatic mechanism for the antithyroid action of thionamides is well documented, literature concerning the effects of thionamides on TPO expression or structure is sparse. Similarly, the molecular basis for the widely exploited immunomodulatory action of thionamides in Graves? disease remains unknown, although many hypotheses have been explored [11, 15, 16]. Bearing in mind that TPO is a universal autoantigen in AITD and that the immune responses are directed against conformational TPO epitopes, we set out to explore the hypothesis that thionamides may influence the structure of TPO (i.e., the conformation of these epitopes) or its subcellular localisation.


Materials and methods
Cell culture, lysis, and protein extraction

A Chinese hamster ovary cell line that had been stably transfected with full-length human TPO (CHO-TPO) was used to generate enzymatically active TPO (a gift from Dr. Monika Gora (Warsaw) and Prof. Paul Banga (London)). CHO-TPO cell monolayers were cultured for 7?10?days in complete media (Hams F12?+?500??g/ml hygromycin B, 10% foetal bovine serum). Cells were washed three times in cold phosphate-buffered saline (PBS) before scraping and pelleting. Cell pellets were resuspended in ice cold lysis buffer (50?mM Tris?HCl, pH?7.3; 150?mM NaCl; 5?mM ethylenediaminetetraacetic acid; 10% glycerol (w/v); 1% Triton X-100 (w/v), protease inhibitors). After two freeze-thaw cycles, debris was pelleted at 13,000 g for 30?min at 4?C. Supernatants were collected as a source of TPO and stored at ?20?C. Aliquots of each preparation were tested for peroxidase activity by oxidation of 0.5% guaiacol in PBS [17].

Immunoblotting

Recombinant human TPO (rhTPO) was prepared as above with the following exceptions: CHO-TPO cells were cultured either for 10?days in the presence of MMI (1, 10, 100, and 500??M) in complete media or for 7?days in complete media followed by 50?h with 6-PTU (Sigma) (10, 100, and 500??M) in serum-free media (OptiMEM). Total protein was standardised by micro-bicinchoninic acid assay (BCA) assay (Pierce labs) and electrophoresed at 20?mA/gel on sodium dodecyl sulphate (SDS)-polyacrylamide gels after mild denaturation by concomitant incubation with 40?mM iodoacetamide and 50?mM dithiothreitol (DTT) in SDS-loading buffer (0.1?M TrisCl, pH?6.8; 20% glycerol, 4% SDS, 0.001% bromophenol blue) for 10?min at 97?C. Alternative denaturation/reduction buffers including DTT only, 2-mercaptoethanol, and iodoacetamide alone were used, as detailed in Fig.?1. Gels were blotted by electro-transfer onto nitrocellulose (Hybond ECL, GE Healthcare) for 90?min at 90?V, on ice. After transfer, non-specific binding to the membrane was blocked using 5% non-fat milk in Tris-buffered saline and 0.1% Tween 20 (TBST) overnight, followed by 16?h incubation with a monoclonal anti-TPO antibody (mAb47, Alexis Biochemicals, UK) in 1% bovine serum albumin (BSA) in phosphate-buffered saline and 0.1% Tween 20 (PBST). Membranes were washed in TBST and incubated for 1?h with anti-mouse IgG alkaline phosphatase conjugate antibody, raised in goat (1/1,000 in 1% BSA in TBST, Pierce) to detect antibody binding. Four 5-min washes followed before adding chemiluminescent substrate (West Dura, Pierce) and exposing to film. Band densitometry was performed using ImageQuant software and normalised for glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression in each lane.

Cloning eGFP/FLAG-TPO

Oligonucleotide primers were designed to flank and overlap the 3? and 5? ends of TPO cDNA, previously cloned into pcDNA3.1 (gift of Dr. B Rapoport, LA [18]), and introduced a G to C base change into the stop codon to allow read-through to C-terminally tagged fusion protein, as well as XbaI and EcoRI sites for subsequent in-frame cloning into pCMV14-3xFLAG vector (Sigma) or AgeI and EcoRI for pEGFP-N1 (Clontech). The full-length cDNA was polymerase chain reaction (PCR)-amplified using high-fidelity Phusion polymerase (NEB, Beverly, MA, USA). The resulting 3?kb bands were sub-cloned into either the TOPO-pCR-XL (Invitrogen) or pGEM T-easy vectors (Promega) and ligated into their final vectors. Single clones positive for full-length TPO were identified by restriction digest and in-frame integration of the cDNA confirmed by DNA sequencing. FLAG-tagged bacterial alkaline phosphatase (FLAG-BAP) and unconjugated enhanced green fluorescent protein (eGFP; pEGFP-N1) constructs were obtained from manufacturers. The unconjugated eGFP construct will be referred to as eGFP-nil.

Co-immunoprecipitation

CHO cells at 70% confluence were co-transfected with FLAG-TPO, eGFP-TPO (10??g per 10?cm2 plate), or their respective controls: FLAG-BAP, eGFP-nil using Fugene HD (Roche, UK). After 18?h, proteins were extracted as previously described. Input samples were retained, and the remainder pre-cleared for non-specific binding with protein-G sepharose beads for 1?h. Samples were then centrifuged at 1,000?g at 4?C for 1?min, the N-terminally tagged TPO fusion proteins were immunoprecipitated overnight using their corresponding antibodies: Anti-FLAG M2 (Sigma), 0.5??g, or GFP(FL) and GFP(B2; Santa Cruz Antibodies, CA, USA). Immunoprecipitating beads were thoroughly washed with lysis buffer and subjected to SDS-polyacrylamide gel electrophoresis (PAGE; as above) alongside their corresponding pre-immunoprecipitated input samples. Immunoblotting was performed as before with the following exceptions: the presence of eGFP-TPO was detected with anti-GFP murine monoclonal or FLAG-TPO using anti-FLAG M2.

Tagged-TPO ELISA

Extracts from CHO cells co-transfected with FLAG-TPO, and either eGFP-TPO or eGFP-nil were prepared as above. Samples with equal total protein, assessed by micro-BCA, were incubated overnight in enzyme-linked-immunosorbent assay (ELISA) plates pre-coated with anti-FLAG M2 mouse antibody (100?ng/well) and blocked with 2% BSA in PBST. After thorough washing with PBST, GFP(FL) rabbit antibody was added for 1?h and detected with anti-rabbit AP-conjugate. Development of para-nitrophenylphosphate (pNPP) was measured (A405) after 1?h.

Bacterial expression of Fab fragments

Four cloned Fabs, TR1.9 and TR1.8; and WR1.7 and SP1.4, which recognised the human TPO B and A immunodominant epitopes, respectively, were obtained (kind gift of Drs. B Rapoport and S McLachlan, LA) [19]. Starter cultures of XL1-blue Escherichia coli containing each of the Fab expression constructs were used to inoculate Superbroth: 30?g/l tryptone, 20?g/l yeast extract, 10?g/l 3-(N-morpholino)propanesulfonic acid, 20?mM MgCl2, 1% glucose grown at 37?C with shaking. At A595 approximately 0.4 Fab production was induced with 1?mM isopropyl ?-D-1-thiogalactopyranoside for 16?h at 28?C. Cells were pelleted and after four freeze-thaw cycles were centrifuged at 30,000?g for 30 min at 4?C. The supernatant was retained as a source of Fab.

ELISA for TPO antibody binding

mAb47 was used to capture recombinant TPO from CHO-TPO cell extracts. Binding of Fabs and patient sera to captured TPO was studied in different MMI (Sigma Chemicals, Poole, UK) conditions, with MMI added after Fab or serum binding. Sera that were positive for TPO antibodies on a conventional chemiluminescent assay (ADVIA Centaur, Bayer) from 36 patients with AITD were studied including 30 (29 females) with Graves? disease, and six (five females) with autoimmune hypothyroidism (mean age 47?years, range 18?78). All sera were collected with the approval of the Northumberland Tyne and Wear local research ethics committee (Reference number 666). Ninety-six well ELISA plates (Nunc, maxisorp) were coated with 3??g mAb47 in carbonate coating buffer per well, overnight. After 3? 5?min PBS 0.1% Tween (PBST) washes, wells were blocked with 2% BSA in PBST for 1?h and incubated overnight with CHO-TPO cell extracts in 2?mg/ml BSA in PBS. Wells were again washed and incubated overnight with either Fabs (diluted 1/1,000) or Graves? patient sera, individually diluted to 1/500 in 2?mg/ml BSA in PBST. To study the effect of MMI, wells were incubated with MMI (500?nM-500?mM) for 30 min at 37?C. Fab or serum Ig binding was detected with antihuman IgG, Fab-specific Alk-Phos Conjugate raised in goat (Sigma). Absorbance (A405) was measured after 1?h incubation pNPP ELISA substrate (Sigma), using a 96-well-plate spectrophotometer (Ascent Multiskan).

Immunocytochemistry

All microscopy was performed using an Axioplan 2 widefield microscope with Zeiss Axiovision software. Cells were seeded after trypsin-passage onto (1.8?cm2) chamberslides (Nunc) and grown to near confluence (approximately 2?3?days). Media was changed to include MMI in complete media as appropriate and cultured for a further 48?h. Culture medium was then removed and cells immediately fixed with fresh 4% paraformaldehyde (PFA) on ice for 15?min. Wells were washed three times with PBS?+?100?mM glycine to remove residual PFA. After culture and fixation, appropriate wells were permeablised for 15?min with PBST (1% Tween-20). All wells were then blocked with 3% BSA in PBS for 1?h before incubation with primary antibody in 1% BSA (1/50 mAb47) for a further hour. Wells were washed three times with PBS and incubated with anti-mouse IgG fluorescein isothiocyanate-conjugate (Sigma) for 1?h. Wells were further washed three times, chambers removed, and vector shield with 4?,6-diamidino-2-phenylindole (Vector Laboratories, UK) applied beneath a glass-coverslip.

Statistical analysis

Groups of measurements were compared using two-tailed t tests, paired by the day of experiment.


Results
High-molecular weight TPO isoforms exist in CHO-TPO cells

Immunoblotting of extracts from the stably transfected CHO-TPO cell line with the monoclonal anti-TPO antibody mAb47 revealed a discrete 110?kDa band and additional high-molecular weight (HMW) bands in the 220?240?kDa range. These HMW complexes were resistant to denaturation with conventional reducing agents (Fig.?1). The 110?kDa isoform is consistent with the expected size of monomeric TPO. As MPO is known to function as a homodimer, we suspected that the HMW-TPO isoforms could also be homodimers.

High-molecular weight TPO is a homodimer

FLAG- and eGFP-tagged TPO were transiently co-expressed in CHO cells, and cell extracts were immunoprecipitated using antibody-conjugated protein-G sepharose beads. Co-transfections using FLAG-BAP and eGFP-nil, along with the tagged TPO constructs were used as controls. Cell extracts from cells co-transfected with FLAG- and eGFP-TPO revealed an eGFP reactive band following immunoprecipitation with anti-FLAG coated beads (Fig.?2). Similarly, immunoprecipitation of the same extracts with anti-GFP coated beads showed an immunoreactive band to anti-FLAG antibody. Co-transfected cells with either FLAG-BAP or eGFP-nil showed no non-specific binding (Fig.?2).

We also looked to confirm TPO dimerisation in an ELISA system, using cell extracts from FLAG-TPO and eGFP-TPO co-transfected CHO cells. Plates were coated with anti-FLAG antibody, to capture FLAG-tagged TPO and probed with anti-GFP to detect TPO dimer. Extracts from FLAG-TPO and eGFP-nil co-transfected cells were used as a control. There was a clear detection of the GFP-tagged TPO when extracts of the FLAG-TPO and eGFP-tagged TPO co-transfected cells were studied (Fig.?3). Whereas, extracts from FLAG-TPO but eGFP-nil co-transfected cells showed low signal when probed with anti-GFP Ab. These results confirm that TPO homodimerises in this system and provides the likely explanation for the HMW-TPO isoforms seen on immunoblotting (Fig.?1).

Thionamide reduces high-molecular weight TPO expression in CHO-TPO cells

CHO-TPO cells were cultured in MMI or PTU, and cell extracts were subject to PAGE and immunoblotting. As before, the anti-TPO antibody mAb47 revealed bands of 110 and 220?240?kDa from untreated CHO-TPO cells (Fig.?4). Cells grown in MMI for 10?days showed a reproducible and significant reduction in the HMW-TPO isoform(s). At an MMI concentration of 1??M, there was a 58% reduction in HMW-TPO bands compared to untreated cells (p?=?0.01), as normalised for GAPDH expression. Greater reductions in these HMW isoforms were found at higher MMI concentrations (58?82% reduction, all p?<?0.05; Fig.?4). In contrast, there was no change in expression of the 110?kDa TPO isoform during MMI treatment. The reduction in TPO-HMW isoforms could be observed after 24?h of culture in MMI 100??M and persisted for at least 10?days of continuous MMI exposure (data not shown).

As PTU is avidly protein bound, we cultured CHO-TPO cells in serum-free media with or without PTU to ensure maximal penetration into the cells. During 50?h exposure of CHO-TPO cells to PTU in serum-free conditions, we observed a similar, substantial reduction in HMW-TPO isoforms of between 34% and 74% across a PTU concentration gradient from 10 to 500??M (p?<?0.01 at 100 and 500??M; Fig.?4).

Effect of methimazole (MMI) on recombinant thyroid peroxidase epitope in vitro

We wished to see whether thionamide effects on TPO structure could alter its interaction with anti-TPO antibodies. An ELISA system was developed to examine antibody binding to TPO by capturing rhTPO from CHO-TPO cell extracts with mAb47. Binding of sera from 36 TPO antibody positive AITD patients and from six TPO antibody negative healthy controls was examined in MMI concentrations from 500?nM to 500?mM. There was no effect of increasing MMI concentration up to 50?mM on binding of patient or control sera to captured rhTPO (Fig.?5). At the highest MMI concentration (500?mM), there appeared to be a direct, possibly ionic, effect of the MMI on retention of antibody-TPO complex on the plate (washing and re-probing with mAb47 revealed no signal). Four Fabs, TR1.9, TR1.8, WR1.7, SP1.4, two each specific for the TPO immunodominant A or B regions, were tested in the ELISA system. Similar to the results with patient sera, we observed no substantial change in Fab binding to anchored TPO after treatment with MMI (500 nM to 50?mM; Fig.?5).

Effect of MMI on TPO localisation in CHO-TPO cells

Membrane localisation has been proposed as the site of enzymatically active mature TPO. We wondered whether thionamide could affect TPO localisation. CHO-TPO cells were grown in MMI at various pharmacologically relevant concentrations and studied by immunocytochemistry using mAb47. TPO expression, frequently in a punctate pattern, was seen both in a juxta-cell membrane location and in the cytosol in untreated CHO-TPO cells (Fig.?6). We observed no change in TPO distribution following exposure to MMI, either in unpermeabilised or permeabilised cells.


Discussion

We set out to explore the hypothesis that thionamides may influence the structure of TPO or its subcellular localisation. On Western analysis of stably transfected CHO-TPO cells, we observed 220?240?kDa HMW-TPO bands, as well as the expected 110?kDa monomeric band (Fig.?1). Although it is established that MPO, in its mature form, exists as a homodimer [20, 21] and it was suspected that TPO would similarly exist in a homodimeric state [22], our co-immunoprecipitation and ELISA binding studies with tagged recombinant TPOs (Figs.?2 and 3) confirm that TPO does indeed form dimeric complexes, at least in this system. Furthermore, we made the novel observation that these dimeric TPO isoforms are dramatically reduced by thionamide treatment, including both MMI and PTU, suggesting that these compounds interfere with the normal structural configuration of TPO. In vivo, thionamides accumulate in the human thyroid, with MMI reaching concentrations of 15??M during therapy for Graves? disease [23]. The response to MMI that we observed at in vitro concentrations as low as 1??M mean that this effect on TPO dimerisation is certainly plausible in vivo at the pharmacological concentrations that are used in Graves? disease. Although data on PTU concentrations in human thyroid is scanty, based on the equivalent potency of PTU to MMI, the higher concentrations of PTU at which we observed a similar reduction in HMW-TPO bands are also consistent with a relevant pharmacological effect in vivo. Nevertheless, our data on the PTU effect are less robust, as prolonged culture in serum-free conditions was not possible, and the intracellular concentration of PTU is likely to have been substantially influenced by protein binding.

In this study, we provide evidence that TPO exists in a homodimeric state and demonstrate that this dimerisation is sensitive to thionamide treatment. Therefore, we wondered if these observations could have relevance for thyroid physiology or the treatment of thyroid diseases. Firstly, it is possible that part of the inhibitory action of thionamides on TPO enzymatic activity in vivo is mediated by this drug-induced change in structure. While this is certainly feasible, it is already known that the hemi-MPO components of disassociated MPO dimer retain their activity and that lactoperoxidase (LPO) exists and functions exclusively in a monomeric state [20, 24]. A second interesting hypothesis is that a change in the structure of the TPO conformational epitope could be responsible for the immunomodulatory effects of thionamide compounds in Graves? disease. This is an attractive hypothesis for several reasons. Firstly, TPO antibodies are practically universal in AITDs, are known to recognise distinct and conformational epitopes [3?6], and are able to mediate direct thyrocyte damage by fixing complement [25], with a plausible role in perpetuation of the immune attack in Graves? disease. Furthermore, thionamide compounds have established immunomodulatory effects in rodent autoimmune thyroiditis. Amelioration of thyroid infiltration and reduction of thyroid antibody production have been demonstrated in several models, including that induced by thyroglobulin in the mouse, by neonatal thymectomy in the Buffalo rat, and in spontaneous lymphocytic thyroiditis in the BB/W rat [26?29]. These observations that thionamide compounds have immunomodulatory properties in autoimmune thyroiditides other than Graves? disease suggest that their immunomodulatory effect may be independent of hyperthyroidism and thus independent of the immune response to the TSHR. Lastly, because of the well-established interaction of thionamides with TPO (responsible for the antithyroid action), the principle of ?Occam?s razor? favours that the immunomodulatory action could be an additional consequence of the same TPO-thionamide interaction. Nevertheless, we cannot exclude that we are simply observing a non-specific reducing effect of thionamides on TPO dimeric isoforms and that similar chronic effects on other multimeric proteins might be responsible for immunomodulation through a mechanism unrelated to TPO. However, this does not seem a likely explanation given the low micromolar concentrations of MMI with which we are able to demonstrate an influence on TPO structure.

Although it is clear that stimulating anti-TSHR antibodies are necessarily present in hyperthyroid Graves? disease, it is possible that they are neither sufficient nor the single aetiological cause of the condition. For instance, neonatal hyperthyroidism caused solely by transplacental passage of TSHR stimulating antibodies is a self-limiting disease without perpetuation of hyperthyroidism. In contrast to anti-TSHR antibodies, which typically circulate at nanograms per millilitre quantities in Graves? disease, anti-TPO antibodies are found at 1,000-fold higher concentration [1]. In addition, anti-TPO antibodies have the capacity to fix complement and to mediate thyrocyte damage through antibody-dependent cell-mediated cytotoxicity [25, 30, 31]. Tantalisingly, there is already evidence that sera from AITD patients may preferentially recognise HMW-TPO isoforms [32, 33]. Thus, our finding that thionamides reduce TPO homodimerisation has the potential to be mechanistically significant. This idea that an autoimmune disease could be abrogated by a drug-induced structural change to an autoantigen has existing mechanistic parallels. It is already well accepted that an immune response can be generated against neoantigen formed by the interaction of a drug with a native protein, for example, in penicillin-induced autoimmune haemolytic anaemia [34]. The proposed mechanism is simply the complementary situation, in that the thionamide-TPO interaction could, in a proportion of individuals, disrupt the TPO conformational epitope to which the autoimmune response has already been directed. This ?removal? of an auto-epitope could lead to a reduced immune response and a subsequent amelioration of the disorder. It should also be noted that many autoantigens are enzymes or cell-surface receptors. These proteins already exist in at least two structural configurations, and thus their epitopes may naturally vary in structure depending upon the state of the receptor (liganded?unliganded) or enzyme (oxidised?reduced). Alternatively, the relative flexibility of their molecular structures may make them particularly susceptible to transformation into a novel conformational state following interaction with a drug.

Our ELISA experiments, using recombinant human TPO bound to the solid phase by mAb47 did not appear to show any effect of MMI on AITD serum or Fab binding to TPO in vitro. This may simply be that the drug effect requires dynamically folding nascent TPO proteins to disrupt the formation of the proper tertiary and quaternary structure. Thus, these effects may not be present in vitro. Alternatively, dimeric (HMW) TPO may be poorly bound to the ELISA plate, or the TPO epitope structure may have been stabilised by its binding to the anchoring mAb47 antibody. Similarly, we could find no evidence to support the straightforward possibility that in the presence of thionamide, TPO becomes mislocalised away from the cell surface. This finding is consistent with a previous study using MMI applied to native thyrocytes [35].

Our studies advance the field by demonstrating a molecular effect of thionamides on TPO macromolecular structure. We demonstrate, for the first time, that HMW-TPO isoforms, which are likely to be dimeric TPO, are decreased in the presence of thionamide in vitro. This observation has possible relevance to a molecular mechanism for both the antithyroid thionamide action and for the immunomodulation of thyroid autoimmunity by these drugs. Additional work is needed both in vitro and in vivo to further illuminate these observations. The possibility of being able to design a novel antithyroid drug with greater immunomodulatory properties than the currently available thionamides, or of being able to predict relapse or remission of Graves? disease following drug treatment, based on a knowledge of antibody repertoire, would have substantial clinical implications.


Acknowledgements

The authors thank Drs. Basil Rapoport and Sandy McLachlan for generously giving their advice and reagents in the conduct of these experiments. We also thank Dr. Monika Gora and Prof. J Paul Banga for kindly providing the CHO-TPO cell line, Prof. Jeremy Lakey for advice about structural biology, Tarana Singh-Dang for technical advice on co-immunoprecipitation, and Anna Mitchell for helpful comments on the manuscript. This work was funded by an MRC studentship to DOM.

Conflict of interest statement The authors declare that they have no conflict of interests.

Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.


References
1.. Beever K,Bradbury J,Phillips D,McLachlan SM,Pegg C,Goral A,Overbeck W,Feifel G,Smith BR. Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidaseClin Chem 1989;35:1949–1954.
1.. Beever K, Bradbury J, Phillips D, McLachlan SM, Pegg C, Goral A, Overbeck W, Feifel G, Smith BR (1989) Highly sensitive assays of autoantibodies to thyroglobulin and to thyroid peroxidase. Clin Chem 35:1949?1954 [pmid: 2776323]
2.. Libert F,Ruel J,Ludgate M,Swillens S,Alexander N,Vassart G,Dinsart C. Thyroperoxidase, an auto-antigen with a mosaic structure made of nuclear and mitochondrial gene modulesEMBO J 1987;6:4193–4196.
2.. Libert F, Ruel J, Ludgate M, Swillens S, Alexander N, Vassart G, Dinsart C (1987) Thyroperoxidase, an auto-antigen with a mosaic structure made of nuclear and mitochondrial gene modules. EMBO J 6:4193?4196 [pmid: 3443105]
3.. Bresson D,Cerutti M,Devauchelle G,Pugniere M,Roquet F,Bes C,Bossard C,Chardes T,Peraldi-Roux S. Localization of the discontinuous immunodominant region recognized by human anti-thyroperoxidase autoantibodies in autoimmune thyroid diseasesJ Biol Chem 2003;278:9560–9569. [doi: 10.1074/jbc.M211930200]
3.. Bresson D, Cerutti M, Devauchelle G, Pugniere M, Roquet F, Bes C, Bossard C, Chardes T, Peraldi-Roux S (2003) Localization of the discontinuous immunodominant region recognized by human anti-thyroperoxidase autoantibodies in autoimmune thyroid diseases. J Biol Chem 278:9560?9569 [pmid: 12501244]
4.. Guo J,McLachlan SM,Rapoport B. Localization of the thyroid peroxidase autoantibody immunodominant region to a junctional region containing portions of the domains homologous to complement control protein and myeloperoxidaseJ Biol Chem 2002;277:40189–40195. [doi: 10.1074/jbc.M205524200]
4.. Guo J, McLachlan SM, Rapoport B (2002) Localization of the thyroid peroxidase autoantibody immunodominant region to a junctional region containing portions of the domains homologous to complement control protein and myeloperoxidase. J Biol Chem 277:40189?40195 [pmid: 12167622]
5.. Gora M,Gardas A,Wiktorowicz W,Hobby P,Watson PF,Weetman AP,Sutton BJ,Banga JP. Evaluation of conformational epitopes on thyroid peroxidase by antipeptide antibody binding and mutagenesisClin Exp Immunol 2004;136:137–144. [doi: 10.1111/j.1365-2249.2004.02422.x]
5.. Gora M, Gardas A, Wiktorowicz W, Hobby P, Watson PF, Weetman AP, Sutton BJ, Banga JP (2004) Evaluation of conformational epitopes on thyroid peroxidase by antipeptide antibody binding and mutagenesis. Clin Exp Immunol 136:137?144 [pmid: 15030525]
6.. Bresson D,Rebuffat S,Peraldi-Roux S. Localization of the immunodominant region on human thyroid peroxidase in autoimmune thyroid diseases: an updateJ Autoimmune Dis 2005;2:2. [doi: 10.1186/1740-2557-2-2]
6.. Bresson D, Rebuffat S, Peraldi-Roux S (2005) Localization of the immunodominant region on human thyroid peroxidase in autoimmune thyroid diseases: an update. J Autoimmune Dis 2:2 [pmid: 15769293]
7.. Abramowicz MJ,Targovnik HM,Varela V,Cochaux P,Krawiec L,Pisarev MA,Propato FV,Juvenal G,Chester HA,Vassart G. Identification of a mutation in the coding sequence of the human thyroid peroxidase gene causing congenital goiterJ Clin Invest 1992;90:1200–1204. [doi: 10.1172/JCI115981]
7.. Abramowicz MJ, Targovnik HM, Varela V, Cochaux P, Krawiec L, Pisarev MA, Propato FV, Juvenal G, Chester HA, Vassart G (1992) Identification of a mutation in the coding sequence of the human thyroid peroxidase gene causing congenital goiter. J Clin Invest 90:1200?1204 [pmid: 1401057]
8.. Astwood E. Chemotherapy of hyperthyroidismHarvey Lect 1945;40:195–235.
8.. Astwood E (1945) Chemotherapy of hyperthyroidism. Harvey Lect 40:195?235
9.. Taurog A. The mechanism of action of the thioureylene antithyroid drugsEndocrinology 1976;98:1031–1046. [doi: 10.1210/endo-98-4-1031]
9.. Taurog A (1976) The mechanism of action of the thioureylene antithyroid drugs. Endocrinology 98:1031?1046 [pmid: 1278093]
10.. Solomon DH,Beck JC,Vanderlaan WP. Prognosis of hyperthyroidism treated by antithyroid drugsJ Am Med Assoc 1953;152:201–205.
10.. Solomon DH, Beck JC, Vanderlaan WP (1953) Prognosis of hyperthyroidism treated by antithyroid drugs. J Am Med Assoc 152:201?205 [pmid: 13044497]
11.. Weetman AP,McGregor AM,Hall R. Evidence for an effect of antithyroid drugs on the natural history of Graves? diseaseClin Endocrinol (Oxf) 1984;21:163–172. [doi: 10.1111/j.1365-2265.1984.tb03456.x]
11.. Weetman AP, McGregor AM, Hall R (1984) Evidence for an effect of antithyroid drugs on the natural history of Graves? disease. Clin Endocrinol (Oxf) 21:163?172 [pmid: 6205795]
12.. Reinwein D,Benker G,Lazarus JH,Alexander WD. A prospective randomized trial of antithyroid drug dose in Graves? disease therapy. European Multicenter Study Group on Antithyroid Drug TreatmentJ Clin Endocrinol Metab 1993;76:1516–1521. [doi: 10.1210/jc.76.6.1516]
12.. Reinwein D, Benker G, Lazarus JH, Alexander WD (1993) A prospective randomized trial of antithyroid drug dose in Graves? disease therapy. European Multicenter Study Group on Antithyroid Drug Treatment. J Clin Endocrinol Metab 76:1516?1521 [pmid: 8501160]
13.. McGregor AM,Petersen MM,McLachlan SM,Rooke P,Smith BR,Hall R. Carbimazole and the autoimmune response in Graves? diseaseN Engl J Med 1980;303:302–307.
13.. McGregor AM, Petersen MM, McLachlan SM, Rooke P, Smith BR, Hall R (1980) Carbimazole and the autoimmune response in Graves? disease. N Engl J Med 303:302?307 [pmid: 6247656]
14.. Marcocci C,Chiovato L,Mariotti S,Pinchera A. Changes of circulating thyroid autoantibody levels during and after the therapy with methimazole in patients with Graves? diseaseJ Endocrinol Investig 1982;5:13–19.
14.. Marcocci C, Chiovato L, Mariotti S, Pinchera A (1982) Changes of circulating thyroid autoantibody levels during and after the therapy with methimazole in patients with Graves? disease. J Endocrinol Investig 5:13?19 [pmid: 6896520]
15.. Weetman AP. The immunomodulatory effects of antithyroid drugsThyroid 1994;4:145–146. [doi: 10.1089/thy.1994.4.145]
15.. Weetman AP (1994) The immunomodulatory effects of antithyroid drugs. Thyroid 4:145?146 [pmid: 7522682]
16.. Volpe R. Evidence that the immunosuppressive effects of antithyroid drugs are mediated through actions on the thyroid cell, modulating thyrocyte-immunocyte signaling: a reviewThyroid 1994;4:217–223. [doi: 10.1089/thy.1994.4.217]
16.. Volpe R (1994) Evidence that the immunosuppressive effects of antithyroid drugs are mediated through actions on the thyroid cell, modulating thyrocyte-immunocyte signaling: a review. Thyroid 4:217?223 [pmid: 7522684]
17.. Hosoya T,Kondo Y,Ui N. Peroxidase activity in thyroid gland and partial purification of the enzymeJ Biochem 1962;52:180–189.
17.. Hosoya T, Kondo Y, Ui N (1962) Peroxidase activity in thyroid gland and partial purification of the enzyme. J Biochem 52:180?189 [pmid: 13964156]
18.. Magnusson RP,Chazenbalk GD,Gestautas J,Seto P,Filetti S,DeGroot LJ,Rapoport B. Molecular cloning of the complementary deoxyribonucleic acid for human thyroid peroxidaseMol Endocrinol 1987;1:856–861. [doi: 10.1210/mend-1-11-856]
18.. Magnusson RP, Chazenbalk GD, Gestautas J, Seto P, Filetti S, DeGroot LJ, Rapoport B (1987) Molecular cloning of the complementary deoxyribonucleic acid for human thyroid peroxidase. Mol Endocrinol 1:856?861 [pmid: 3153466]
19.. Nishikawa T,Costante G,Prummel MF,McLachlan SM,Rapoport B. Recombinant thyroid peroxidase autoantibodies can be used for epitopic ?fingerprinting? of thyroid peroxidase autoantibodies in the sera of individual patientsJ Clin Endocrinol Metab 1994;78:944–949. [doi: 10.1210/jc.78.4.944]
19.. Nishikawa T, Costante G, Prummel MF, McLachlan SM, Rapoport B (1994) Recombinant thyroid peroxidase autoantibodies can be used for epitopic ?fingerprinting? of thyroid peroxidase autoantibodies in the sera of individual patients. J Clin Endocrinol Metab 78:944?949 [pmid: 7512572]
20.. Andrews PC,Krinsky NI. The reductive cleavage of myeloperoxidase in half, producing enzymically active hemi-myeloperoxidaseJ Biol Chem 1981;256:4211–4218.
20.. Andrews PC, Krinsky NI (1981) The reductive cleavage of myeloperoxidase in half, producing enzymically active hemi-myeloperoxidase. J Biol Chem 256:4211?4218 [pmid: 6260790]
21.. Fiedler TJ,Davey CA,Fenna RE. X-ray crystal structure and characterization of halide-binding sites of human myeloperoxidase at 1.8. A resolutionJ Biol Chem 2000;275:11964–11971. [doi: 10.1074/jbc.275.16.11964]
21.. Fiedler TJ, Davey CA, Fenna RE (2000) X-ray crystal structure and characterization of halide-binding sites of human myeloperoxidase at 1.8. A resolution. J Biol Chem 275:11964?11971 [pmid: 10766826]
22.. Hamada N,Portmann L,DeGroot LJ. Characterization and isolation of thyroid microsomal antigenJ Clin Invest 1987;79:819–825. [doi: 10.1172/JCI112889]
22.. Hamada N, Portmann L, DeGroot LJ (1987) Characterization and isolation of thyroid microsomal antigen. J Clin Invest 79:819?825 [pmid: 3818950]
23.. Jansson R,Dahlberg PA,Johansson H,Lindstrom B. Intrathyroidal concentrations of methimazole in patients with Graves? diseaseJ Clin Endocrinol Metab 1983;57:129–132. [doi: 10.1210/jcem-57-1-129]
23.. Jansson R, Dahlberg PA, Johansson H, Lindstrom B (1983) Intrathyroidal concentrations of methimazole in patients with Graves? disease. J Clin Endocrinol Metab 57:129?132 [pmid: 6687892]
24.. Singh AK,Singh N,Sharma S,Singh SB,Kaur P,Bhushan A,Srinivasan A,Singh TP. Crystal structure of lactoperoxidase at 2.4. A resolutionJ Mol Biol 2008;376:1060–1075. [doi: 10.1016/j.jmb.2007.12.012]
24.. Singh AK, Singh N, Sharma S, Singh SB, Kaur P, Bhushan A, Srinivasan A, Singh TP (2008) Crystal structure of lactoperoxidase at 2.4. A resolution. J Mol Biol 376:1060?1075 [pmid: 18191143]
25.. Khoury EL,Hammond L,Bottazzo GF,Doniach D. Presence of the organ-specific ?microsomal? autoantigen on the surface of human thyroid cells in culture: its involvement in complement-mediated cytotoxicityClin Exp Immunol 1981;45:316–328.
25.. Khoury EL, Hammond L, Bottazzo GF, Doniach D (1981) Presence of the organ-specific ?microsomal? autoantigen on the surface of human thyroid cells in culture: its involvement in complement-mediated cytotoxicity. Clin Exp Immunol 45:316?328 [pmid: 6172224]
26.. Davies TF,Weiss I,Gerber MA. Influence of methimazole on murine thyroiditis. Evidence for immunosuppression in vivoJ Clin Invest 1984;73:397–404. [doi: 10.1172/JCI111225]
26.. Davies TF, Weiss I, Gerber MA (1984) Influence of methimazole on murine thyroiditis. Evidence for immunosuppression in vivo. J Clin Invest 73:397?404 [pmid: 6699171]
27.. Cohen SB,Weetman AP. Antithyroid drugs ameliorate thymectomy-induced experimental autoimmune thyroiditisAutoimmunity 1988;1:51–58.
27.. Cohen SB, Weetman AP (1988) Antithyroid drugs ameliorate thymectomy-induced experimental autoimmune thyroiditis. Autoimmunity 1:51?58 [pmid: 2856510]
28.. Rennie DP,McGregor AM,Keast D,Weetman AP,Foord SM,Dieguez C,Williams ED,Hall R. The influence of methimazole on thyroglobulin-induced autoimmune thyroiditis in the ratEndocrinology 1983;112:326–330. [doi: 10.1210/endo-112-1-326]
28.. Rennie DP, McGregor AM, Keast D, Weetman AP, Foord SM, Dieguez C, Williams ED, Hall R (1983) The influence of methimazole on thyroglobulin-induced autoimmune thyroiditis in the rat. Endocrinology 112:326?330 [pmid: 6847827]
29.. Allen EM,Rajatanavin R,Nogimori T,Cushing G,Ingbar SH,Braverman LE. The effect of methimazole on the development of spontaneous lymphocytic thyroiditis in the diabetes-prone BB/W ratAm J Med Sci 1986;292:267–271. [doi: 10.1097/00000441-198611000-00003]
29.. Allen EM, Rajatanavin R, Nogimori T, Cushing G, Ingbar SH, Braverman LE (1986) The effect of methimazole on the development of spontaneous lymphocytic thyroiditis in the diabetes-prone BB/W rat. Am J Med Sci 292:267?271 [pmid: 3777011]
30.. Bogner U,Schleusener H,Wall JR. Antibody-dependent cell mediated cytotoxicity against human thyroid cells in Hashimoto?s thyroiditis but not Graves? diseaseJ Clin Endocrinol Metab 1984;59:734–738. [doi: 10.1210/jcem-59-4-734]
30.. Bogner U, Schleusener H, Wall JR (1984) Antibody-dependent cell mediated cytotoxicity against human thyroid cells in Hashimoto?s thyroiditis but not Graves? disease. J Clin Endocrinol Metab 59:734?738 [pmid: 6548225]
31.. Guo J,Jaume JC,Rapoport B,McLachlan SM. Recombinant thyroid peroxidase-specific Fab converted to immunoglobulin G (IgG) molecules: evidence for thyroid cell damage by IgG1, but not IgG4, autoantibodiesJ Clin Endocrinol Metab 1997;82:925–931. [doi: 10.1210/jc.82.3.925]
31.. Guo J, Jaume JC, Rapoport B, McLachlan SM (1997) Recombinant thyroid peroxidase-specific Fab converted to immunoglobulin G (IgG) molecules: evidence for thyroid cell damage by IgG1, but not IgG4, autoantibodies. J Clin Endocrinol Metab 82:925?931 [pmid: 9062508]
32.. Kaufman KD,Rapoport B,Seto P,Chazenbalk GD,Magnusson RP. Generation of recombinant, enzymatically active human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto?s thyroiditisJ Clin Invest 1989;84:394–403. [doi: 10.1172/JCI114179]
32.. Kaufman KD, Rapoport B, Seto P, Chazenbalk GD, Magnusson RP (1989) Generation of recombinant, enzymatically active human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto?s thyroiditis. J Clin Invest 84:394?403 [pmid: 2474568]
33.. Baker JR,Arscott P,Johnson J. An analysis of the structure and antigenicity of different forms of human thyroid peroxidaseThyroid 1994;4:173–178. [doi: 10.1089/thy.1994.4.173]
33.. Baker JR, Arscott P, Johnson J (1994) An analysis of the structure and antigenicity of different forms of human thyroid peroxidase. Thyroid 4:173?178 [pmid: 7919999]
34.. Levine BB. Immunologic mechanisms of penicillin allergy. A haptenic model system for the study of allergic diseases of manN Engl J Med 1966;275:1115–1125.
34.. Levine BB (1966) Immunologic mechanisms of penicillin allergy. A haptenic model system for the study of allergic diseases of man. N Engl J Med 275:1115?1125 [pmid: 5923027]
35.. Aguayo J,Michaud P,Iitaka M,Degroot LJ,Row VV,Volpe R. Lack of effect of methimazole on thyrocyte cell-surface antigen expressionAutoimmunity 1989;2:133–143. [doi: 10.3109/08916938909019950]
35.. Aguayo J, Michaud P, Iitaka M, Degroot LJ, Row VV, Volpe R (1989) Lack of effect of methimazole on thyrocyte cell-surface antigen expression. Autoimmunity 2:133?143 [pmid: 2577490]

Figures

[Figure ID: Fig1]
Fig.?1 

Recombinant human TPO forms high molecular weight complexes. Cell extracts, native (A) or denatured (B; 98?C for 10 min) with either 2% 2-ME, 50 mM DTT, 40 mM iodoacetamide (IAA), or 50 mM DTT and 40 mM IAA, from CHO cells stably-expressing rhTPO were assessed by immunoblotting with mAb47. Bands migrating at ~105-110 kDa were observed in all conditions corresponding to monomeric TPO. High molecular weight TPO isoforms were also detected above 200 kDa



[Figure ID: Fig2]
Fig.?2 

Differentially tagged recombinant human thyroid peroxidase (rhTPO) forms dimeric complexes in Chinese hamster ovary (CHO) cells. CHO cells were co-transfected with FLAG- and enhanced green fluorescent protein (eGFP)-TPO, or their respective control constructs FLAG-BAP or eGFP-nil. Cell extracts were immunoprecipitated via their fusion-tags using either anti-FLAG M2, anti-GFP B2, or anti-GFP FL antibody bound to protein-G sepharose. a FLAG-BAP (a.) and FLAG-TPO (b.) were immunoprecipitated using the anti-FLAG sepharose. Anti-GFP detected co-immunoprecipitating bands in the FLAG-TPO transfected cell extract but not following FLAG-BAP transfection (c.). b Both GFP antibodies co-immunoprecipitated FLAG-TPO along with eGFP-TPO (d. and f.) but not in cell extracts transfected with the eGFP-nil control construct (e. and g.)



[Figure ID: Fig3]
Fig.?3 

Demonstration of dimeric thyroid peroxidase (TPO) by tagged TPO enzyme-linked-immunosorbent assay. FLAG-TPO from Chinese hamster ovary cells co-transfected with FLAG-TPO and enhanced green fluorescent protein (eGFP)-TPO, or eGFP-nil constructs was captured onto solid phase by murine anti-FLAG M2. A rabbit anti-GFP (FL) antibody detected eGFP-TPO with an increase in the A405 of >900% (mean of three replicates). This optical density was significantly greater than that observed in the absence of GFP antibody (p?<?0.01) or than that of the GFP-nil control (p?<?0.01)



[Figure ID: Fig4]
Fig.?4 

High-molecular weight (HMW)-thyroid peroxidase (TPO) isoforms are reduced in Chinese hamster ovary (CHO)-TPO cultured with thionamide. Immunoblotting with mAb47 compared levels of recombinant human TPO (rhTPO) isoforms from CHO-TPO cells cultured in the presence of (a) methimazole (MMI) for 10?days or (c) propylthiouracil (PTU) for 50?h immediately prior to harvest. HMW (>220?kDa) band densities were quantitated from triplicate experiments and normalised as a percentage of that observed in the absence of thionamide, relative to each sample?s glyceraldehyde 3-phosphate dehydrogenase for both (b) MMI and (d) PTU. Highly significant reductions in HMW-TPO occurred at all MMI concentrations compared to the untreated condition (*p???0.01, **p?<?0.05). Likewise, lower levels of HMW isoforms were observed at all concentrations of PTU compared to the untreated condition



[Figure ID: Fig5]
Fig.?5 

Effect of methimazole (MMI) incubation on antibody binding to thyroid peroxidase (TPO). The effect of MMI on the binding of autoimmune thyroid disease (AITD) sera or immunodominant region (IDR)-specific Fabs to recombinant human TPO (rhTPO) was investigated by sandwich enzyme-linked-immunosorbent serologic assay incorporating a 30-min MMI incubation (500?nM?500?mM) following application of the sera or Fab. Substrate absorbance (measured as A405) was normalised as a percentage of the maximum observed for each serum or Fab, after non-specific binding to untransfected Chinese hamster ovary extracts was subtracted. a Representative absorbance data, from a single experiment, with six AITD patient sera and a single control. b Normalised data for 36 AITD patient sera and six normal control sera were included. No significant decrease (two-tailed t test) in binding of Graves? or control sera at MMI concentrations up to 50?mM was observed. c Four Fabs, specific for the TPO IDR were studied in triplicate. Following incubation with MMI at concentrations of 500?nM?50?mM, there were no alterations in Fab binding to rhTPO. Substrate absorbance was dramatically reduced (p???0.01) at 500?mM MMI in all experiments (we believe a non-specific effect, possibly related to high ionic content; see ?Results?). Data presented as means with bars representing SEM



[Figure ID: Fig6]
Fig.?6 

Effect of methimazole (MMI) on localisation of thyroid peroxidase (TPO) in stably transfected Chinese hamster ovary (CHO) cells. CHO-TPO cells were seeded on coverslips and grown until near confluence, when in the media was changed to include/exclude MMI (5, 100, and 500??M). After 24?h, cells were fixed and left unpermeablised (a) or permeablised (b) with 0.1% Triton X-100 for 30?min. TPO was detected using mab47 and visualised with anti-mouse IgG fluorescein isothiocyanate (green) conjugate at ?100 magnification. Vectorshield mounting agent contained 4?,6-diamidino-2-phenylindole to counterstain DNA (blue). Untransfected CHO cells were included as a control (c). TPO showed juxta-cell membrane and cytosolic localization, in a punctate pattern. There was no major change in TPO localisation at any MMI concentration tested



Article Categories:
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Keywords: Keywords Thyroid peroxidase, Homodimerisation, Methimazole, Propylthiouracil, Graves? disease, Immunomodulation.

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