Document Detail

Thyroid hormone transport in a human glioma cell line.
MedLine Citation:
PMID:  2328825     Owner:  NLM     Status:  MEDLINE    
The uptake of 3,5,3'-triiodothyronine (T3) and thyroxine (T4) was studied in human glioma cells (Hs 683) and compared with that in several other neural cell lines. At 25 degrees C or 37 degrees C, total cell uptake rose rapidly and reached equilibrium within 60 min. The glioma cells had the highest uptake: 47.6 fmol of L-T3 and 43.4 fmol of L-T4 per 10(6) cells at 37 degrees C. These were inhibited 77% and 72%, respectively, by excess unlabeled hormone. Uptake in the nuclei reached equilibrium between 90 and 120 min and was also highest in glioma cells: 1.46 fmol of L-T3 and 0.49 fmol of L-T4 per 10(6) cells. When expressed as percent of total cell uptake, however, glioma cells had the lowest values (3.1% for L-T3 and 1.1% for L-T4). Also in contrast to other cell lines, glioma cells transported L-T4 almost as effectively as L-T3. D-T3 and D-T4 total cell uptake was 86% and 96% lower than that of the respective L-isomers, and the nuclear uptake as a fraction of the cell uptake was similar. Kinetic analysis of the initial rate of cell uptake gave Vmax values for D-T3 and D-T4 that were 97% and 98% lower than for the L-isomers. Antimycin and monodansylcadaverine decreased the Vmax as well as the equilibrium cell and nuclear uptake of the L-isomers. The apparent nuclear affinity constant for L-T4 in intact cells was inhibited 90% in the presence of antimycin, whereas no effect was observed in isolated nuclei.(ABSTRACT TRUNCATED AT 250 WORDS)
E Goncalves; M Lakshmanan; A Pontecorvi; J Robbins
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Molecular and cellular endocrinology     Volume:  69     ISSN:  0303-7207     ISO Abbreviation:  Mol. Cell. Endocrinol.     Publication Date:  1990 Mar 
Date Detail:
Created Date:  1990-05-31     Completed Date:  1990-05-31     Revised Date:  2013-02-11    
Medline Journal Info:
Nlm Unique ID:  7500844     Medline TA:  Mol Cell Endocrinol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  157-65     Citation Subset:  IM    
Clinical Endocrinology Branch, National Institute of Diabetes, Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.
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MeSH Terms
Aminoisobutyric Acids / pharmacology
Antimycin A / analogs & derivatives,  pharmacology
Binding, Competitive
Biological Transport / drug effects
Cadaverine / analogs & derivatives,  pharmacology
Cell Membrane / metabolism
Cell Nucleus / metabolism
Glioma / metabolism*
Medulloblastoma / metabolism
Neuroblastoma / metabolism
Ouabain / pharmacology
Thyroxine / metabolism*
Triiodothyronine / metabolism*
Tumor Cells, Cultured
Reg. No./Substance:
0/Aminoisobutyric Acids; 10121-91-2/monodansylcadaverine; 11118-72-2/antimycin; 1E7ZW41IQU/2-aminoisobutyric acid; 462-94-2/Cadaverine; 630-60-4/Ouabain; 642-15-9/Antimycin A; 6893-02-3/Triiodothyronine; 7488-70-2/Thyroxine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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