| Thyroid hormone action: early calorigenic effect on dispersed rat liver cells in the absence of protein synthesis. | |
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MedLine Citation:
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PMID: 3391135 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Isolated dispersed rat liver cells were prepared by hypothyroid Sprague-Dawley rats. The cells were incubated under 95% O2/5% CO2 in Krebs-Ringer-bicarbonate buffer at pH 7.3-7.4 at 37 degrees C. The medium had been enriched with 2% bovine serum albumin (previously stripped of thyroid hormone) and 5-10 mM alanine as substrate. Two hour incubations were carried out with or without added triiodothyronine (T3) at 3 nM or 300-1,000 nM concentrations. Oxygen consumption determined at the end of the period of incubation with the Clark oxygen electrode showed stimulation above control values in the hormone treated flasks; parallel studies in which cycloheximide (100 microM) had been added to cells to block protein synthesis also showed enhanced oxygen consumption in response to T3. The results indicated a response to the hormone not dependent on new protein formation. |
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Authors:
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K Sterling; G Tsuboyama; M A Brenner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrine research Volume: 14 ISSN: 0743-5800 ISO Abbreviation: Endocr. Res. Publication Date: 1988 |
Date Detail:
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Created Date: 1988-08-18 Completed Date: 1988-08-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8408548 Medline TA: Endocr Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 109-16 Citation Subset: IM |
Affiliation:
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Department of Medicine, Columbia University College of Physicians and Surgeons, New York, NY. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Cycloheximide / pharmacology Liver / drug effects*, metabolism Oxygen Consumption / drug effects Protein Biosynthesis Rats Rats, Inbred Strains Triiodothyronine / pharmacology* |
| Grant Support | |
ID/Acronym/Agency:
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DK-37870/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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66-81-9/Cycloheximide; 6893-02-3/Triiodothyronine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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