Document Detail


Thyroid regeneration: characterization of clear cells after partial thyroidectomy.
MedLine Citation:
PMID:  22454152     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although having the capacity to grow in response to a stimulus that perturbs the pituitary-thyroid axis, the thyroid gland is considered not a regenerative organ. In this study, partial thyroidectomy (PTx) was used to produce a condition for thyroid regeneration. In the intact thyroid gland, the central areas of both lobes served as the proliferative centers where microfollicles, and bromodeoxyuridine (BrdU)-positive and/or C cells, were localized. Two weeks after PTx, the number of BrdU-positive cells and cells with clear or faintly eosinophilic cytoplasm were markedly increased in the central area and continuous to the cut edge. Clear cells were scant in the cytoplasm, as determined by electron microscopy; some retained the characteristics of calcitonin-producing C cells by having neuroendocrine granules, whereas others retained follicular cell-specific features, such as the juxtaposition to a lumen with microvilli. Some cells were BrdU-positive and expressed Foxa2, the definitive endoderm lineage marker. Serum TSH levels drastically changed due to the thyroidectomy-induced acute reduction in T(4)-generating tissue, resulting in a goitrogenesis setting. Microarray followed by pathway analysis revealed that the expression of genes involved in embryonic development and cancer was affected by PTx. The results suggest that both C cells and follicular cells may be altered by PTx to become immature cells or immature cells that might be derived from stem/progenitor cells on their way to differentiation into C cells or follicular cells. These immature clear cells may participate in the repair and/or regeneration of the thyroid gland.
Authors:
Takashi Ozaki; Tsutomu Matsubara; Daekwan Seo; Minoru Okamoto; Kunio Nagashima; Yoshihito Sasaki; Suguru Hayase; Tsubasa Murata; Xiao-Hui Liao; Jeffrey Hanson; Jaime Rodriguez-Canales; Snorri S Thorgeirsson; Kennichi Kakudo; Samuel Refetoff; Shioko Kimura
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural     Date:  2012-03-27
Journal Detail:
Title:  Endocrinology     Volume:  153     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-04-23     Completed Date:  2012-06-29     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2514-25     Citation Subset:  AIM; IM    
Affiliation:
Laboratory of Metabolism, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Female
Male
Mice
Regeneration / physiology*
Thyroid Gland / cytology,  physiology*,  surgery
Thyroidectomy*
Grant Support
ID/Acronym/Agency:
1Z01BC005522/BC/NCI NIH HHS; DK 15070/DK/NIDDK NIH HHS; R37 DK015070/DK/NIDDK NIH HHS
Comments/Corrections

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