| Thyroid Hormones Influence Human Dendritic Cells' Phenotype, Function, and Subsets Distribution. | |
| | |
MedLine Citation:
|
PMID: 21190445 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Background: Dendritic cells (DCs) are the most effective antigen-presenting cells and key regulators of immune response. The immunoregulatory properties of DCs strongly depend on the microenvironment in which DCs have been matured and activated. Thyroid hormones are an important part of this environment and regulate many vital processes including growth and cellular metabolism. The aim of the study was an analysis of the influence of thyroid hormones on blood DC subtypes ex vivo, including the surface expression of molecules involved in antigen presentation, costimulation, and maturation, as well as on functional properties of DCs in vitro. Methods: Blood samples for the quantitative and phenotypic analysis of peripheral blood plasmacytoid and myeloid DC subtypes were collected from thyroidectomized patients at two time points: (i) at the time of the so-called stimulation with endogenous thyrotropin-a group of hypothyroid patients after l-thyroxine (L-T(4)) withdrawal (pretreatment group)-and (ii) after 2 months of L-T(4) administration for thyrotropin suppression-a posttreatment group. The phenotype of DCs including HLA-DR, costimulatory molecules (CD40, CD80, and CD86), and maturation marker CD83 was assessed by flow cytometry. The influence of isolated peripheral blood DCs on autologous peripheral blood mononuclear cell proliferation and cytokine secretion (interferon alpha, interleukin-12) under triiodothyronine (T(3)) deficiency or T(3) excess was investigated in culture experiments. Results: The percentage of peripheral blood plasmacytoid and myeloid DCs was higher after L-T(4) administration when compared with the pretreatment group. Moreover, the expression of CD86 on both DC subtypes was higher in the L-T(4) treated than in the hypothyroid patients. In the in vitro experiments, T(3) stimulation increased CD86 expression on cultured DCs. The phenotypic difference was paralleled by enhanced ability of T(3)-stimulated DCs to activate interleukin-12 secretion and proliferation of autologous peripheral blood mononuclear cells (PBMLs) in coculture experiments. Conclusions: In the present study, we provide for the first time an evidence that the thyrometabolic status has an influence on the phenotype and function of human peripheral blood DCs. This observation may be of potential importance for the understanding of the pathogenesis of immune and endocrine disorders. |
| | |
Authors:
|
Marek Dedecjus; Mariusz Stasiolek; Jan Brzezinski; Krzysztof Selmaj; Andrzej Lewinski |
Related Documents
:
|
11101875 - Immune suppression and skin cancer development: regulation by nkt cells. 12459545 - The macrolide immunosuppressants in dermatology: mechanisms of action. 20329695 - Protective clothing in the sun. 17522815 - Modified skin window technique for the extended characterisation of acute inflammation ... 12871025 - Fc receptors as potential targets for the treatment of allergy, autoimmune disease and ... 14634285 - Palmitate induces apoptosis via a direct effect on mitochondria. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2010-12-29 |
Journal Detail:
|
Title: Thyroid : official journal of the American Thyroid Association Volume: - ISSN: 1557-9077 ISO Abbreviation: - Publication Date: 2010 Dec |
Date Detail:
|
Created Date: 2010-12-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9104317 Medline TA: Thyroid Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
1 Department of General, Oncological, and Endocrine Surgery, Polish Mother's Memorial Hospital-Research Institute, Medical University of Lodz , Lodz, Poland . |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Prevalence, clinicopathologic features, and somatic genetic mutation profile in familial versus spor...
Next Document: Outcome of Very Long-Term Treatment with Antithyroid Drugs in Graves' Hyperthyroidism Associated wit...