Document Detail

Thyroid cell growth: sphingomyelin metabolism as non-invasive marker for cell damage acquired during spaceflight.
MedLine Citation:
PMID:  21087161     Owner:  NLM     Status:  In-Process    
Prolonged spaceflights are known to elicit changes in human cardiovascular, musculoskeletal, and nervous systems, whose functions are regulated by the thyroid gland. It is known that sphingomyelin metabolism is involved in apoptosis (programmed cell death) of thyroid cells induced by UVC radiation, but at present no data exists with regard to this phenomenon, which occurs during space missions. The aim of this study was to analyze, for the first time, the effect of spaceflight on the enzymes of sphingomyelin metabolism, sphingomyelinase, and sphingomyelin synthase, and to determine whether the ratio between the two enzymes might be used as a possible marker for thyroid activity during space missions. Both quiescent thyroid cells and thyroid cells stimulated to proliferate with thyrotropin (TSH) were cultured during the Eneide and Esperia missions on the International Space Station. The results show that during space missions the cells treated with TSH grew only 1.5 ± 0.65-fold and, thus, behave similarly to quiescent cells, while on the ground the same cells, maintained in experimental conditions that reproduced those of the flight, grew 7.71 ± 0.67-fold. Comparison of the sphingomyelinase/sphingomyelin-synthase ratio and the levels of Bax, STAT3, and RNA polymerase II in proliferating, quiescent, pro-apoptotic, or apoptotic cells demonstrated that thyroid cells during space missions were induced into a pro-apoptotic state. Given its specificity and the small amount of cells needed for analysis, we propose the use of the sphingomyelinase/sphingomyelin-synthase ratio as a marker of functional status of thyroid cells during space missions. Further studies could lead to its use in real time during prolonged spaceflights.
Elisabetta Albi; Saverio Ambesi-Impiombato; Maristella Villani; Ilaria De Pol; Renza Spelat; Remo Lazzarini; Giuseppina Perrella
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Astrobiology     Volume:  10     ISSN:  1557-8070     ISO Abbreviation:  Astrobiology     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-11-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101088083     Medline TA:  Astrobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  811-20     Citation Subset:  IM    
Department of Clinical and Experimental Medicine, Physiopathology Section, University School of Medicine, Perugia, Italy.
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