| Thyroglobulin may affect telomerase activity in thyroid follicular cells. | |
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MedLine Citation:
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PMID: 18830915 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Telomerase (TA) activity is known to be present in malignant tumor cells, but not in most somatic differentiated cells. TA shows relatively high activity in thyroid cancer cells, but reports vary. This fact prompted us to elucidate whether cell component inhibitors of TA in the thyroid follicles can modulate its activity. The activity of TA extracted from Hela cells was inhibited by mixing with the supernatant fraction of human thyroid tissue extract. To examine the effect of iodine, thyroid hormones (l-T3 and l-T4) and human thyroglobulin (hTg) contained in the thyroid follicles, l-T3, l-T4 and hTg were added to the TRAP assay system in vitro, using TA from Hela cells. Iodine, l-T3 and l-T4 did not affect TA activity, but hTg inhibited the TA activity in a dose-dependent manner (IC(50) of hTg: ca 0.45 microM: inhibiting concentration of hTg was from 0.15 microM to 3.0 microM). The hTg inhibition was not evident in the RT-PCR system, suggesting no effect of hTg on Taq DNA polymerase activity. The hTg inhibition of TA activity was attenuated by dNTP but not significantly by TS primer. These data suggest that hTg contained in thyroid follicular cells of various thyroid diseases may affect the TA activity measured in biopsied thyroid specimens, and that the reduction of the TA activity by hTg may induce slow progression and growth, and low grade malignancy of thyroid cancer, particularly differentiated carcinoma. |
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Authors:
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Akio Nagasaka; Naohisa Oda; Akira Nakai; Keiko Hotta; Mutsuko Nagata; Taiya Kato; Atsushi Suzuki; Mitsuyasu Itoh; Hitoshi Miura; Motoo Hakuta; Shonen Yoshida; Yatsuka Hibi; Katsumi Iwase |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of enzyme inhibition and medicinal chemistry Volume: 24 ISSN: 1475-6374 ISO Abbreviation: J Enzyme Inhib Med Chem Publication Date: 2009 Apr |
Date Detail:
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Created Date: 2009-03-16 Completed Date: 2009-08-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101150203 Medline TA: J Enzyme Inhib Med Chem Country: England |
Other Details:
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Languages: eng Pagination: 524-30 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine, Fujita Health University School of Medicine, Toyoake, Aichi, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Carcinoma, Papillary, Follicular
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enzymology,
metabolism Dose-Response Relationship, Drug Hela Cells Humans Reverse Transcriptase Polymerase Chain Reaction Telomerase / metabolism* Thyroglobulin / pharmacology* Thyroid Gland / enzymology*, metabolism Thyroid Neoplasms / enzymology, metabolism Time Factors |
| Chemical | |
Reg. No./Substance:
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9010-34-8/Thyroglobulin; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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