Document Detail


Thymosins β-4 and β-10 are expressed in bovine ovarian follicles and upregulated in cumulus cells during meiotic maturation.
MedLine Citation:
PMID:  20883646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
β-Thymosins are small proteins that regulate the actin cytoskeleton and are involved in cell motility, differentiation, the induction of metalloproteinases, in anti-inflammatory processes and tumourigenesis. However, their roles in the ovary have not yet been elucidated. Using transcriptomics and real time reverse transcription-polymerase chain reaction validation, the present study demonstrates that thymosin β-4 (TMSB4) and thymosin β-10 (TMSB10) are upregulated in bovine cumulus cells (CCs) during in vitro maturation of cumulus-oocyte complexes (COCs) in parallel with an increase in mRNA expression of HAS2, COX2 and PGR genes. Using immunocytochemistry, both proteins were found to be localised mainly in granulosa cells, CCs and oocytes, in both the cytoplasm and nucleus, as well as being colocalised with F-actin stress fibres in CCs. Using different maturation mediums, we showed that the expression of TMSB10, but not TMSB4, was positively correlated with COC expansion and progesterone secretion and negatively correlated with apoptosis. Immunofluorescence, coupled with terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL), demonstrated the absence of TMSB4 and/or TMSB10 in apoptotic cells. TMSB10 expression was higher in COCs matured in vivo than in vitro, and differences related to the age of the animal were observed. TMSB4 and/or TMSB10 expression was unchanged, whereas HAS2 overexpressed in CCs from oocytes that developed to the blastocyst stage in vitro compared with those that did not. Thus, TMSB4 and/or TMSB10 ovarian expression patterns suggest that these two thymosins may be involved in cumulus modifications during maturation.
Authors:
Mohamad Salhab; Pascal Papillier; Christine Perreau; Catherine Guyader-Joly; Joelle Dupont; Pascal Mermillod; Svetlana Uzbekova
Related Documents :
20047046 - Exogenously expressed human ku70 stabilizes ku80 in xenopus oocytes and induces heterol...
11133166 - Transcriptional activity of the mouse oocyte genome: companion granulosa cells modulate...
17294366 - Isolation of apoptotic mouse fetal oocytes by annexinv assay.
20668366 - Serotonin 2c receptor (5-ht2cr) mrna editing-induced down-regulation of 5-ht2cr functio...
23466456 - Bmp4 reverses multidrug resistance through modulation of bcl-2 and gdnf in glioblastoma.
9770276 - Activation of a consensus fnr-dependent promoter by dnr of pseudomonas aeruginosa in re...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, fertility, and development     Volume:  22     ISSN:  1031-3613     ISO Abbreviation:  Reprod. Fertil. Dev.     Publication Date:  2010  
Date Detail:
Created Date:  2010-10-04     Completed Date:  2010-12-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8907465     Medline TA:  Reprod Fertil Dev     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  1206-21     Citation Subset:  IM    
Affiliation:
INRA, UMR85 Physiologie de la Reproduction et des Comportements, CNRS,UMR6175, Université de Tours, Nouzilly, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Apoptosis
Blotting, Western
Cattle
Culture Media / metabolism
Cumulus Cells / metabolism*
Cyclooxygenase 2 / genetics
Embryo Culture Techniques / veterinary
Female
Fertilization in Vitro / veterinary
Fluorescent Antibody Technique
Gene Expression Profiling / methods
Gene Expression Regulation, Developmental
Glucuronosyltransferase / genetics
Immunohistochemistry
In Situ Nick-End Labeling
Meiosis*
Oligonucleotide Array Sequence Analysis
Oogenesis*
Ovarian Follicle / metabolism*
Progesterone / metabolism
RNA, Messenger / metabolism
Receptors, Progesterone / genetics
Reverse Transcriptase Polymerase Chain Reaction
Thymosin / genetics,  metabolism*
Time Factors
Up-Regulation
Chemical
Reg. No./Substance:
0/Culture Media; 0/RNA, Messenger; 0/Receptors, Progesterone; 57-83-0/Progesterone; 61512-21-8/Thymosin; 77591-33-4/thymosin beta(4); 87397-91-9/thymosin beta(10); EC 1.14.99.1/Cyclooxygenase 2; EC 2.4.1.17/Glucuronosyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Retinol-binding protein (RBP), retinol and ?-carotene in the bovine uterus and plasma during the oes...
Next Document:  Oocyte developmental competence is reduced in sows during the seasonal infertility period.