Document Detail


Thymocytes stimulate metabolism of arachidonic acid in rat thymic epithelial cells.
MedLine Citation:
PMID:  2121375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Arachidonic acid metabolites play an important role in the development of T cells in the thymus. In the normal animal, prostaglandin levels in the thymus are significantly higher than in plasma. Herein, we have studied the regulation of arachidonic acid metabolism using the thymic endocrine epithelial cell line, TEA3A1, and thymocytes. We have found that TEA3A1 cells, but not thymocytes, produced prostaglandins and thromboxanes. We have also found that thymocytes could stimulate the production of arachidonic acid metabolites in TEA3A1 cells when both cells were cocultured. The strongest stimulation was observed when TEA3A1 cells were cocultured with thymocyte subpopulations either negative for OX8 or W3/25 functional surface markers (either double-negative or single-positive thymocytes). Furthermore, cell-cell contact seems to be absolutely required for the activation of arachidonic acid metabolism in TEA3A1 cells. The study presented here describes the existence of a novel regulatory mechanism of arachidonic acid metabolism which may play an important role in the development of T cells in the thymus.
Authors:
L Sun; A S Piltch; P S Liu; L A Johnson; J Hayashi
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cellular immunology     Volume:  131     ISSN:  0008-8749     ISO Abbreviation:  Cell. Immunol.     Publication Date:  1990 Nov 
Date Detail:
Created Date:  1990-12-10     Completed Date:  1990-12-10     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  1246405     Medline TA:  Cell Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  86-97     Citation Subset:  IM    
Affiliation:
W. Alton Jones Cell Science Center, Incorporated, Lake Placid, New York 12946.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arachidonic Acid
Arachidonic Acids / metabolism*
Cell Communication
Chromatography, Thin Layer
Epithelium / metabolism
Male
Prostaglandins / biosynthesis
Radioimmunoassay
Rats
Thymus Gland / cytology,  metabolism*,  physiology*
Grant Support
ID/Acronym/Agency:
P01 CA37589/CA/NCI NIH HHS; P01 DK38639/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Arachidonic Acids; 0/Prostaglandins; 506-32-1/Arachidonic Acid

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