Document Detail


THYMINELESS MUTATION SITE ON ESCHERICHIA COLI CHROMOSOME.
MedLine Citation:
PMID:  14137616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Kitsuji, Nobuo (Kanazawa University, Kanazawa, Ishikawa, Japan). Thymineless mutation site on Escherichia coli chromosome. J. Bacteriol. 87:802-808. 1964.-Kinetic studies of the recombinant formation in crosses between Escherichia coli K-12 Hfr H, Hfr C, or Hfr P10 as donor and F(-)W1177thy(-)his(-) as recipients showed that the time of appearance of thy(+)Sm(r) recombinants lay between that of xyl(+)Sm(r) and his(+)Sm(r), indicating that the thy gene lies between the xyl and his markers. Genetic analysis of recombinants of the Hfr C x W117thy(-)his(-) cross further indicated that the thy gene is located on the chromosome between the Sm marker and the his marker. In matings between seven independently isolated thymineless mutants of Hfr P10 and F(-)W1177thy(-)his(-), thy(+) recombinants of various recombination rates appeared. Thus, it was suggested that in E. coli K-12 the thy gene occupies some region between the Sm marker and the his marker on the chromosome, and that there are many thymineless mutation sites in it.
Authors:
N KITSUJI
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  87     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1964 Apr 
Date Detail:
Created Date:  1964-08-01     Completed Date:  1996-12-01     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  802-8     Citation Subset:  OM    
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MeSH Terms
Descriptor/Qualifier:
Bacteriological Techniques*
Chromosomes*
Escherichia coli*
Genetics*
Mutation*
Research*
Thymine*
Chemical
Reg. No./Substance:
65-71-4/Thymine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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