Document Detail

Thymidine secretion by hybridoma and myeloma cells.
MedLine Citation:
PMID:  16472778     Owner:  NLM     Status:  MEDLINE    
Secretion of thymidine appeared to be a common property of hybridoma and myeloma cells, but not of other cell types, which were tested. Of three hybridoma cell lines tested, all secreted thymidine in amounts resulting in the accumulation of thymidine to concentrations of 10-20microM in the culture medium. Also three of five myeloma cell lines that were analyzed secrete thymidine, but none of the other cell types that were studied. Thymidine was purified to homogeneity (4mg purified from 3l of culture medium) and identified as such by nuclear magnetic resonance spectroscopy. The cells that secreted thymidine showed high resistance to the growth inhibitory effect of thymidine.
Bjørn Spilsberg; Frode Rise; Dirk Petersen; Jon Nissen-Meyer
Related Documents :
23586648 - Fundamental study of osteoclast chemotaxis toward chemoattractants expressed in periodo...
25149518 - Use of non-ionic surfactants for improvement of terpene production in saccharomyces cer...
24688178 - Evaluation of a real-time polymerase chain reaction assay of the outer membrane protein...
24958548 - In vitro culture and characterization of spermatogonial stem cells on sertoli cell feed...
6086538 - Pharmacologic modulation in vitro of human histamine-induced suppressor cell activity.
22643728 - Isolation and culture of human fungiform taste papillae cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-02-03
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  342     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-20     Completed Date:  2006-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM    
Department of Molecular Biosciences, University of Oslo, Oslo, Norway.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Line
Cell Proliferation / drug effects
Hybridomas / drug effects,  metabolism,  secretion*
Magnetic Resonance Spectroscopy
Multiple Myeloma / metabolism,  secretion*
Thymidine / biosynthesis,  pharmacology,  secretion*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Novel short chain fatty acids restore chloride secretion in cystic fibrosis.
Next Document:  Arabidopsis cytosolic glutamine synthetase AtGLN1;1 is a potential substrate of AtCRK3 involved in l...