Document Detail


Thymidine kinase (TK) induction after infection of TK-deficient rabbit cell mutants with bovine herpesvirus type 1 (BHV-1): isolation of TK- BHV-1 mutants.
MedLine Citation:
PMID:  6316635     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytosol thymidine kinase (TK) activity is enhanced at 6 hr after bovine embryo tracheal (EBTr) and rabbit skin fibroblast (RAB-9) cells are infected with the Los Angeles and Cooper strains of bovine herpesvirus type 1 (BHV-1). To learn whether this enhancement resulted from the induction of a virus-specific TK activity, biochemical and genetic studies were carried out. The biochemical experiments demonstrated that: (i) the BHV-1-induced TK activity had a relative disc PAGE mobility (Rm) characteristic of other herpesvirus-encoded TKs and distinctly different from the Rm value of the cytosol TK of host cells; and (ii) the BHV-1-induced TK was significantly more sensitive to competitive inhibition by arabinosylthymine (araT) than the cytosol TKs of EBTr and RAB-9 cells. The genetic experiments entailed the isolation of a bromodeoxyuridine (BrdUrd)-resistant rabbit cell line [RAB(BU)] deficient in cytosol TK activity and of BrdUrd- and araT-resistant BHV-1 mutants. RAB(BU) cells acquired TK activity after they were infected by wild-type, TK+ BHV-1, but not drug-resistant BHV-1 mutants. The experiments strongly suggest that wild-type BHV-1 induces a virus-specific TK activity.
Authors:
S Kit; H Qavi
Related Documents :
8263115 - Establishment and characterisation of murine cells constitutively expressing the fusion...
16781755 - Glut-1-independent infection of the glioblastoma/astroglioma u87 cells by the human t c...
2932535 - Location of influenza virus m, np and ns1 proteins in microinjected cells.
2156435 - The detection of epstein-barr virus in hairy cell leukemia cells by in situ hybridization.
12824775 - Nerve growth factor induces proliferation of pc12 cells through cdc42.
18558485 - Checking graphite and stainless anodes with an experimental model of marine microbial f...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  130     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Arabinonucleosides / pharmacology
Cell Line
Cytosol / enzymology
Electrophoresis, Polyacrylamide Gel
Enzyme Induction
Herpesvirus 1, Bovine / enzymology*,  genetics
Mutation
Phosphorylation
Rabbits
Thymidine / analogs & derivatives,  metabolism,  pharmacology
Thymidine Kinase / biosynthesis*
Trifluridine / pharmacology
Grant Support
ID/Acronym/Agency:
1-K6-AI-2352-20/AI/NIAID NIH HHS; CA-06656-20/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Arabinonucleosides; 50-89-5/Thymidine; 605-23-2/ara-T; 70-00-8/Trifluridine; EC 2.7.1.21/Thymidine Kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Identification of a new protein present in vesicular stomatitis virus-infected Chinese hamster ovary...
Next Document:  Sequence of the 5' end of the Sendai virus genome and its variable representation in complementary f...