Document Detail

Thymidine kinase (TK) induction after infection of TK-deficient rabbit cell mutants with bovine herpesvirus type 1 (BHV-1): isolation of TK- BHV-1 mutants.
MedLine Citation:
PMID:  6316635     Owner:  NLM     Status:  MEDLINE    
Cytosol thymidine kinase (TK) activity is enhanced at 6 hr after bovine embryo tracheal (EBTr) and rabbit skin fibroblast (RAB-9) cells are infected with the Los Angeles and Cooper strains of bovine herpesvirus type 1 (BHV-1). To learn whether this enhancement resulted from the induction of a virus-specific TK activity, biochemical and genetic studies were carried out. The biochemical experiments demonstrated that: (i) the BHV-1-induced TK activity had a relative disc PAGE mobility (Rm) characteristic of other herpesvirus-encoded TKs and distinctly different from the Rm value of the cytosol TK of host cells; and (ii) the BHV-1-induced TK was significantly more sensitive to competitive inhibition by arabinosylthymine (araT) than the cytosol TKs of EBTr and RAB-9 cells. The genetic experiments entailed the isolation of a bromodeoxyuridine (BrdUrd)-resistant rabbit cell line [RAB(BU)] deficient in cytosol TK activity and of BrdUrd- and araT-resistant BHV-1 mutants. RAB(BU) cells acquired TK activity after they were infected by wild-type, TK+ BHV-1, but not drug-resistant BHV-1 mutants. The experiments strongly suggest that wild-type BHV-1 induces a virus-specific TK activity.
S Kit; H Qavi
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Virology     Volume:  130     ISSN:  0042-6822     ISO Abbreviation:  Virology     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1984-01-07     Completed Date:  1984-01-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  381-9     Citation Subset:  IM    
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MeSH Terms
Arabinonucleosides / pharmacology
Cell Line
Cytosol / enzymology
Electrophoresis, Polyacrylamide Gel
Enzyme Induction
Herpesvirus 1, Bovine / enzymology*,  genetics
Thymidine / analogs & derivatives,  metabolism,  pharmacology
Thymidine Kinase / biosynthesis*
Trifluridine / pharmacology
Grant Support
1-K6-AI-2352-20/AI/NIAID NIH HHS; CA-06656-20/CA/NCI NIH HHS
Reg. No./Substance:
0/Arabinonucleosides; 50-89-5/Thymidine; 605-23-2/ara-T; 70-00-8/Trifluridine; EC Kinase

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