Document Detail

Thymic stromal lymphopoietin: an immune cytokine gene associated with the metabolic syndrome and blood pressure in severe obesity.
MedLine Citation:
PMID:  22304237     Owner:  NLM     Status:  MEDLINE    
A previous expression profiling of VAT (visceral adipose tissue) revealed that the TSLP (thymic stromal lymphopoietin) gene was less expressed in severely obese men with (n=7) compared with without (n=7) the MetS (metabolic syndrome). We hypothesized that TSLP SNPs (single nucleotide polymorphisms) are associated with TSLP gene expression in VAT and with MetS phenotypes. Following validation of lower TSLP expression (P=0.003) in VAT of severely obese men and women with (n=70) compared with without (n=60) the MetS, a detailed genetic investigation was performed at the TSLP locus by sequencing its promoter, exons and intron-exon splicing boundaries using DNA of 25 severely obese subjects. Five tagging SNPs were genotyped in the 130 subjects from the expression analysis to test whether these SNPs contributed to TSLP expression variability (ANOVAs) and then genotyped in two independent samples of severely obese men (total, n=389) and women (total, n=894). In a sex-stratified multistage experimental design, ANOVAs were performed to test whether tagging SNPs were associated with MetS components treated as continuous variables. We observed that the non-coding SNP rs2289277 was associated with TSLP mRNA abundance (P=0.04), as well as with SBP [systolic BP (blood pressure)] (P=0.004) and DBP (diastolic BP) (P=0.0003) in men when adjusting for age, waist circumference, smoking and medication treating hypertension. These novel observations suggest that TSLP expression in VAT may partly explain the inter-individual variability for metabolic impairments in the presence of obesity and that specific SNPs (rs2289277 and/or correlating SNPs) may influence TSLP gene expression as well as BP in obese men.
Valérie Turcot; Luigi Bouchard; Geneviève Faucher; Véronique Garneau; André Tchernof; Yves Deshaies; Louis Pérusse; Simon Marceau; Simon Biron; Odette Lescelleur; Laurent Biertho; Marie-Claude Vohl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical science (London, England : 1979)     Volume:  123     ISSN:  1470-8736     ISO Abbreviation:  Clin. Sci.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-03-29     Completed Date:  2012-05-11     Revised Date:  2012-06-13    
Medline Journal Info:
Nlm Unique ID:  7905731     Medline TA:  Clin Sci (Lond)     Country:  England    
Other Details:
Languages:  eng     Pagination:  99-109     Citation Subset:  IM    
Institute of Nutraceuticals and Functional Foods, Quebec City, Canada G1V 0A6.
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MeSH Terms
Blood Pressure*
Cytokines / genetics*,  physiology
Metabolic Syndrome X / etiology*,  genetics
Obesity / complications,  genetics*
Polymorphism, Single Nucleotide*
RNA, Messenger / analysis
Grant Support
MOP-209380//Canadian Institutes of Health Research
Reg. No./Substance:
0/Cytokines; 0/RNA, Messenger; 0/thymic stromal lymphopoietin
Erratum In:
Clin Sci (Lond). 2012 Aug 1;123(4):271

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