Document Detail


Thymic humoral factor gamma 2: purification and amino acid sequence of an immunoregulatory peptide from calf thymus.
MedLine Citation:
PMID:  3261994     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Thymic humoral factor gamma 2 (THF-gamma 2), an octapeptide essential for immune regulation, was purified from calf thymus. The purification of THF-gamma 2, monitored in vitro and in vivo in mouse splenocyte proliferation assays, was achieved by gel filtration of low molecular weight thymus extracts followed by ion-exchange chromatography and sequential reversed-phase high-performance liquid chromatography. The process yielded 5 micrograms of THF-gamma 2/1000 kg of thymus tissue. The concentration of THF-gamma 2 required for augmentation of lymphocyte proliferation and interleukin 2 production was 5 ng/mL in vitro and 10 ng/kg per mouse in vivo. THF-gamma 2 has the amino acid sequence Leu-Glu-Asp-Gly-Pro-Lys-Phe-Leu. The proposed structure has been confirmed because a peptide was synthesized on the basis of this sequence that showed activity identical with that of the biological molecule. It shows no homology to the amino acid sequence of other thymic hormones nor is it part of any peptide or protein of known sequence. THF-gamma 2 retains essentially all of the biological activity of the thymus extract from which it is derived.
Authors:
Y Burstein; V Buchner; M Pecht; N Trainin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemistry     Volume:  27     ISSN:  0006-2960     ISO Abbreviation:  Biochemistry     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-10-18     Completed Date:  1988-10-18     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0370623     Medline TA:  Biochemistry     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4066-71     Citation Subset:  IM    
Affiliation:
Department of Organic Chemistry, Weizmann Institute of Science, Rehovot, Israel.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis
Animals
Biological Assay
Cattle
Chromatography, High Pressure Liquid
Interleukin-2 / biosynthesis
Mice
Oligopeptides / isolation & purification*
Thymus Gland / analysis*
Thymus Hormones / isolation & purification*
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Interleukin-2; 0/Oligopeptides; 0/Thymus Hormones; 107489-37-2/thymic humoral factor gamma 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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