Document Detail


Thyme (Thymus vulgaris L.) leaves and its constituents increase the activities of xenobiotic-metabolizing enzymes in mouse liver.
MedLine Citation:
PMID:  16117610     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of thyme (Thymus vulgaris L.) leaves and its phenolic compounds, thymol and carvacrol, on the activities of xenobiotic-metabolizing enzymes, i.e., phase I enzymes such as 7-ethoxycoumarin O-deethylase (ECOD) and phase II enzymes such as glutathione S-transferase (GST) and quinone reductase (QR), were investigated. Mice were fed with a diet containing thyme (0.5% or 2.0%) or treated orally with thymol (50-200 mg/kg) or carvacrol (50-200 mg/kg) once a day for 7 successive days, and then the enzyme activities in the livers were analyzed. Dietary administration of 2% thyme caused slightly but significantly higher ECOD, GST, and QR activities by 1.1-1.4-fold. Thymol (200 mg/kg) treatment resulted in significantly higher ECOD, GST, and QR activities by 1.3-1.9-fold, and carvacrol (200 mg/kg) treatment caused significantly higher ECOD, GST, and QR activities by 1.3-1.7-fold. Thymol-treated animals had significantly higher protein levels of GST alpha and GST micro, and carvacrol-treated animals had significantly higher levels of GST micro. These results imply that thyme contains bifunctional inducers (i.e., substances capable of inducing both phase I and phase II enzymes) and that thymol and carvacrol may account for the effects of thyme.
Authors:
Keiko Sasaki; Keiji Wada; Yoshiko Tanaka; Teruki Yoshimura; Koozi Matuoka; Takahiko Anno
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of medicinal food     Volume:  8     ISSN:  1096-620X     ISO Abbreviation:  J Med Food     Publication Date:  2005  
Date Detail:
Created Date:  2005-08-24     Completed Date:  2005-11-10     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9812512     Medline TA:  J Med Food     Country:  United States    
Other Details:
Languages:  eng     Pagination:  184-9     Citation Subset:  IM    
Affiliation:
Laboratory for Molecular/Cellular Biology, Faculty of Pharmaceutical Sciences, Chiba Institute of Science, Choshi-shi, Chiba, Japan. ksasaki@cis.ac.jp
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MeSH Terms
Descriptor/Qualifier:
7-Alkoxycoumarin O-Dealkylase / metabolism
Animals
Blotting, Western
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Glutathione Transferase / metabolism
Liver / drug effects,  enzymology*
Male
Mice
Monoterpenes / pharmacology*
NAD(P)H Dehydrogenase (Quinone) / metabolism
Organ Size / drug effects
Plant Extracts / pharmacology*
Plant Leaves
Random Allocation
Thymol / pharmacology*
Thymus Plant / chemistry*
Xenobiotics / metabolism*
Chemical
Reg. No./Substance:
0/Monoterpenes; 0/Plant Extracts; 0/Xenobiotics; 499-75-2/carvacrol; 89-83-8/Thymol; EC 1.14.13.-/7-Alkoxycoumarin O-Dealkylase; EC 1.6.5.2/NAD(P)H Dehydrogenase (Quinone); EC 2.5.1.18/Glutathione Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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