| Thy-1 Signals through PPARγ to Promote Lipofibroblast Differentiation in the Developing Lung. | |
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MedLine Citation:
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PMID: 22268140 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Thy-1 is a glycosylphosphytidylinositol linked cell surface glycoprotein present on a subset of lung fibroblasts that has an important role in postnatal alveolarization. In the present study, we define the role of Thy-1 in pulmonary lipofibroblast differentiation and in the regulation of lipid homeostasis via Peroxisome Proliferator-Activated Receptor-γ (PPARγ). Thy-1 was associated with interstitial cells containing lipid droplets in vivo. Transfection of Thy-1 into Thy-1 (-) fibroblasts increased triglyceride content, fatty acid uptake, and the expression of the lipofibroblast marker adipocyte differentiation related protein (ADRP). Thy-1 (+) fibroblasts exhibited 2.4 fold higher PPARγ activity, and inhibition or activation of PPARγ reduced and increased triglyceride content, respectively. Thy-1 (-) fibroblasts were not responsive to either of the PPARγ agonists ciglitazone or Prostaglandin J(2) (PGJ(2)) supporting the importance of Thy-1 in signaling via PPARγ. Thy-1 (+) fibroblasts expressed significantly higher levels of fatty acid transporter protein 3 mRNA, had higher rates of fatty acid uptake and increased triglyceride content. Inhibition of fatty acid transporter protein function reduced Thy-1 (+) fibroblast lipid content. Expression of Thy-1 in C57BL/6 lung fibroblasts increased in the neonatal period, coinciding with the onset of alveolarization. Thy-1 promoted lipofibroblast differentiation via expression of PPARγ, stimulated lipid accumulation via fatty acid esterification, and enhanced fatty acid uptake mediated by fatty acid transporter proteins. Thy-1 is important in the regulation of lipofibroblast differentiation and function in the developing lung. |
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Authors:
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Brian M Varisco; Namasivayam Ambalavanan; Jeffrey A Whitsett; James S Hagood |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: American journal of respiratory cell and molecular biology Volume: - ISSN: 1535-4989 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8917225 Medline TA: Am J Respir Cell Mol Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Cincinnati Children's Hospital Research Foundation, Cincinnati, Ohio, United States. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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