| Thromboxane formation from arachidonic acid and prostaglandin H2 in rabbit spleen. | |
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MedLine Citation:
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PMID: 6404897 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Incubation of [1-14C]arachidonic acid (AA) and [1-14C]prostaglandin (PG)H2 with rabbit spleen homogenate and microsomes resulted in the formation of a substance with the chromatographic properties of thromboxane (Tx)B2. The radiolabeled material was indistinguishable from authentic TxB2 on TLC in three solvent systems and on radiometric gas chromatography. The generation of TxB2-like material from AA and PGH2 was not observed after boiling of the homogenate and microsomes, and was completely inhibited by imidazole (5 mM). The transformation of AA into the TxB2-like material was not observed during incubation in the presence of indomethacin (28 microM). These results indicate that TxB2 is the principal product of arachidonic acid metabolism by the homogenate or microsomes of rabbit spleen. |
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Authors:
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T Hidaka; T Ueta; R Ogura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of biochemistry Volume: 93 ISSN: 0021-924X ISO Abbreviation: J. Biochem. Publication Date: 1983 Feb |
Date Detail:
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Created Date: 1983-06-17 Completed Date: 1983-06-17 Revised Date: 2007-12-19 |
Medline Journal Info:
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Nlm Unique ID: 0376600 Medline TA: J Biochem Country: JAPAN |
Other Details:
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Languages: eng Pagination: 367-73 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Arachidonic Acid Arachidonic Acids / metabolism* Carbon Radioisotopes / diagnostic use Kinetics Male Microsomes / metabolism Prostaglandin Endoperoxides / metabolism* Prostaglandin Endoperoxides, Synthetic / metabolism* Prostaglandin H2 Prostaglandins H / metabolism* Rabbits Spleen / metabolism* Thromboxane B2 / biosynthesis* Thromboxanes / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Arachidonic Acids; 0/Carbon Radioisotopes; 0/Prostaglandin Endoperoxides; 0/Prostaglandin Endoperoxides, Synthetic; 0/Prostaglandins H; 0/Thromboxanes; 42935-17-1/Prostaglandin H2; 506-32-1/Arachidonic Acid; 54397-85-2/Thromboxane B2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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