Document Detail

Thromboxane (TX)A3 and prostaglandin (PG)I3 are formed in man after dietary eicosapentaenoic acid: identification and quantification by capillary gas chromatography-electron impact mass spectrometry.
MedLine Citation:
PMID:  2996649     Owner:  NLM     Status:  MEDLINE    
The low incidence of myocardial infarction in Greenland Eskimos has been related to their traditional marine diet rich in eicosapentaenoic acid. However, whether dietary eicosapentaenoic acid is indeed transformed in man to antiaggregatory PGI3 and weakly proaggregatory TXA3 has not been clarified. In our studies we ingested either cod liver oil or mackerel both rich in eicosapentaenoic acid. Formation of TXB3, the hydrolysis product of TXA3, in platelet-rich plasma stimulated ex vivo with collagen was traced by capillary GC/EIMS. Via external standard, TXB3 formation in platelets was estimated to be 5-15% of TXB2 formation. From urine we extracted dinor metabolites of PGI according to a selective method. We utilized delta 17-2,3-dinor-6-keto-PGF1 alpha (PGI3-M) as an index of total body production of PGI3 in analogy to 2,3-dinor-6-keto-PGF1 alpha (PGI2-M), the major urinary metabolite of PGI2. We separated PGI2-M and PGI3-M as the Me, MO, Me3Si derivatives by capillary gas chromatography and identified PGI3-M by EI mass spectrometry. Excretion of PGI3-M, which was not detectable under control conditions, was 83 +/- 25 ng/24 h (SD) after ingestion of cod liver oil and 134 +/- 38 ng/24 h after mackerel ingestion, while excretion of PGI2-M was 162 +/- 52 ng/24 h and 236 +/- 32 ng/24 h, respectively. Our findings with diets rich in EPA show that it is possible in man to change in vivo the spectrum of biologically active prostanoids by nutritional means and alter it in a favourable direction.
S Fischer; P C Weber
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomedical mass spectrometry     Volume:  12     ISSN:  0306-042X     ISO Abbreviation:  Biomed. Mass Spectrom.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-12-18     Completed Date:  1985-12-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0430246     Medline TA:  Biomed Mass Spectrom     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  470-6     Citation Subset:  IM    
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MeSH Terms
Blood Platelets / metabolism
Eicosapentaenoic Acid / administration & dosage,  metabolism*
Epoprostenol / metabolism*
Gas Chromatography-Mass Spectrometry
Thromboxanes / metabolism*
Reg. No./Substance:
0/Thromboxanes; 1553-41-9/Eicosapentaenoic Acid; 35121-78-9/Epoprostenol; 60114-68-3/thromboxane A3; 68794-57-0/prostaglandin I3

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