Document Detail

Thrombogenic properties of murine anti-cardiolipin antibodies induced by beta 2 glycoprotein 1 and human immunoglobulin G antiphospholipid antibodies.
MedLine Citation:
PMID:  8840870     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Recurrent arterial thrombosis and venous thrombosis are frequent complications of the antiphospholipid syndrome (APS). Patients produce anti-cardiolipin antibodies, but the role of these antibodies in thrombus formation is uncertain. This study used a unique CD-1 mouse model of thrombosis to determine whether anti-cardiolipin and anti-beta 2 glycoprotein 1 (beta 2 GP1) antibodies induced immunologically in these animals are thrombogenic. METHODS AND RESULTS: The CD-1 mouse model enables measurement of the kinetics of a thrombus induced in the femoral vein of the animal. Animals are first anesthetized, then one femoral vein is exposed and subjected to a standardized, nonpenetrating "pinch" injury that induces a thrombus. The vein is trans-illuminated, and the growing thrombus is visualized on a television screen. The rate of formation and disappearance of the thrombus as well as its area can be measured by a computer attached to the television. Three groups of CD-1 mice (each group comprising seven animals) were studied. Group 1 mice were actively immunized with beta 2GP1, resulting in production of anti-beta 2GP1 and anti-cardiolipin antibodies. Group 2 mice were actively immunized with human immunoglobulin G (IgG) anti-cardiolipin antibodies and produced anti-human IgG as well as anti-cardiolipin antibodies (the latter by an idiotype-anti-idiotype reaction). These animals did not produce anti-beta 2GP1 antibodies. Group 3 mice were immunized with human serum albumin (HSA) and produced anti-HSA but not anti-cardiolipin antibodies. The kinetics of thrombus formation induced in the femoral veins of the experimental mice were compared. Results showed that the mean thrombus area as well as mean time during which thrombi persisted were significantly greater in group 1 and group 2 mice compared with group 3. There was no statistical difference between group 1 or group 2. CONCLUSIONS: Demonstration of a thrombogenic effect of murine anti-cardiolipin antibodies suggests that these antibodies may be pathogenic in humans with APS.
S S Pierangeli; S W Liu; G Anderson; J H Barker; E N Harris
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Circulation     Volume:  94     ISSN:  0009-7322     ISO Abbreviation:  Circulation     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-11-29     Completed Date:  1996-11-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0147763     Medline TA:  Circulation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1746-51     Citation Subset:  AIM; IM    
Antiphospholipid Standardization Laboratory, Morehouse School of Medicine, Atlanta, GA 30310, USA.
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MeSH Terms
Antibodies, Anticardiolipin / immunology*,  physiology*
Antibodies, Antiphospholipid / immunology*
Antibody Formation
Glycoproteins / immunology*
Immunoglobulin G / immunology*
Mice, Inbred Strains
Serum Albumin / immunology
Thrombosis / etiology*
beta 2-Glycoprotein I
Reg. No./Substance:
0/Antibodies, Anticardiolipin; 0/Antibodies, Antiphospholipid; 0/Glycoproteins; 0/Immunoglobulin G; 0/Serum Albumin; 0/beta 2-Glycoprotein I

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