Document Detail


Thrombin induces thromboplastin synthesis in cultured vascular endothelial cells.
MedLine Citation:
PMID:  2417348     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cultured human umbilical vein endothelial cells responded to thrombin (10(-2) - 10 NIH u/ml) with a 2-5 fold increase in thromboplastin activity. The maximum response was reached after 4 hr in serum-free medium. The effect of thrombin was fully inhibited by the presence of 50% (v/v) fetal calf serum or more in the medium, by preincubation of thrombin with hirudin or by treatment of thrombin with N-bromosuccinimide or phenylmethylsulfonyl fluoride. The thrombin-induced thromboplastin activity was inhibited by incubation of the cells with cycloheximide (2 micrograms/ml) or actinomycin D (2 micrograms/ml) showing that the response depended on de novo protein and RNA synthesis. It was also suppressed by exposure of the cells to two different phosphodiesterase inhibitors, 3-butyl-1-methyl-xanthine (5 X 10(-4) M) and rac-4 (3-butoxy-4-methoxybenzyl)-2-imidazole (5 X 10(-4) M), to the transmethylation inhibitors 3-deazaadenosine (10(-5) M) and 1-homocysteine thiolactone (2 X 10(-5) M) in combination and to the intracellular calcium antagonist 8-(N,N-diethylamino)-octyl 3,4,5,-tri-methoxybenzoate hydrochloride (8 X 10(-5) M). Our results suggest that small amounts of thrombin can induce thromboplastin synthesis in endothelial cells in vitro and that this synthesis probably is regulated by the intracellular level of cAMP, by cytoplasmic Ca2+ and possibly also by transmethylation reactions.
Authors:
K S Galdal; T Lyberg; S A Evensen; E Nilsen; H Prydz
Related Documents :
6948808 - The effects of nicotine on mouse first molar tooth germs in organ culture.
7271278 - Effects of low penicillin concentrations on cell morphology and on peptidoglycan and pr...
6981168 - Activity of trimethoprim-sulfamethoxazole against chlamydia trachomatis in vitro.
10529838 - Production of tnf-alpha by primary cultures of human keratinocytes challenged with loxo...
8020808 - Serum free medium increases expression of markers of differentiation in human colonic c...
9788538 - Effect of reg protein on rat pancreatic ductal cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thrombosis and haemostasis     Volume:  54     ISSN:  0340-6245     ISO Abbreviation:  Thromb. Haemost.     Publication Date:  1985 Aug 
Date Detail:
Created Date:  1986-01-28     Completed Date:  1986-01-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7608063     Medline TA:  Thromb Haemost     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  373-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
1-Methyl-3-isobutylxanthine / pharmacology
Cells, Cultured
Cycloheximide / pharmacology
Dactinomycin / pharmacology
Endothelium / cytology,  drug effects,  metabolism*
Fetal Blood / physiology
Hirudins / pharmacology
Humans
Thrombin / antagonists & inhibitors,  pharmacology*
Thromboplastin / biosynthesis*
Umbilical Veins / cytology
Chemical
Reg. No./Substance:
0/Hirudins; 28822-58-4/1-Methyl-3-isobutylxanthine; 50-76-0/Dactinomycin; 66-81-9/Cycloheximide; 9035-58-9/Thromboplastin; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Immunochemical study of the alpha 2-glycoprotein from the atherosclerotically altered aortic wall in...
Next Document:  Effects of adrenaline infusion on platelet number, volume and release reaction.