| Thrombin increases expression of urokinase receptor by activation of the thrombin receptor. | |
| | |
MedLine Citation:
|
PMID: 7558719 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
PURPOSE: To investigate the effect of thrombin on the urokinase plasminogen activator receptor (u-PAR) in retinal pigment epithelial (RPE) cells. METHODS: The authors analyzed u-PAR mRNA by Northern blot hybridization. Retinal pigment epithelial cell surface u-PAR was assayed by measuring the amount of functional urokinase plasminogen activator (u-PA) bound to cells at saturation. Retinal pigment epithelial cells were derived from fetal retinal tissue and established in primary cell culture. RESULTS: Thrombin increased u-PAR mRNA 4-fold in RPE cells examined by Northern blot hybridization, whereas the amount of thrombin receptor mRNA was unchanged. Thrombin stimulated u-PA binding to RPE cells 2.5- to 5-fold in a time- and dose-dependent manner. Hirudin, a thrombin antagonist, completely blocked the effects of thrombin on u-PAR expression in RPE cells. Phosphatidylinositol phospholipase C treatment of RPE cells resulted in the abolition of thrombin-induced u-PA binding. Recombinant soluble u-PAR competitively inhibited two-chain u-PA binding to the surface of thrombin-treated RPE cells. A thrombin receptor agonist peptide (SFLLRNPNDKYEPF) also induced a 2.5-fold increase in binding of u-PA to the surface of RPE cells. CONCLUSION: Thrombin increases u-PAR expression by RPE cells by a mechanism involving activation of the seven transmembrane thrombin receptor. |
| | |
Authors:
|
Z Yang; R L Cohen; G M Lui; D A Lawrence; M A Shuman |
Related Documents
:
|
12963729 - Biochemical and molecular characterization of two phosphatidic acid-selective phospholi... 293739 - Analysis of genetic complementation by whole-cell microtechniques in fibroblast heterok... 47369 - A hemolytic plaque assay for the detection of direct and indirect antibody-forming cell... 3549899 - Apparent sensitivity of human k lymphocytes to the spatial orientation and organization... 194839 - Effector cell involved in cell-mediated cytotoxicity to cells infected with herpes simp... 22783109 - Occurrence of clubroot on shepherd's-purse caused by plasmodiophora brassicae. 20869589 - Neural progenitor nuclei in motion. 224209 - Hepatocyte suspensions and cultures as tools in experimental carcinogenesis. 23594289 - Immunohistochemical analysis of the role and relationship between notch-1 and oct-4 exp... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Investigative ophthalmology & visual science Volume: 36 ISSN: 0146-0404 ISO Abbreviation: Invest. Ophthalmol. Vis. Sci. Publication Date: 1995 Oct |
Date Detail:
|
Created Date: 1995-11-06 Completed Date: 1995-11-06 Revised Date: 2010-03-24 |
Medline Journal Info:
|
Nlm Unique ID: 7703701 Medline TA: Invest Ophthalmol Vis Sci Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 2254-61 Citation Subset: IM |
Affiliation:
|
Cancer Research Institute, University of California at San Francisco 94143-0128, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Blotting, Northern Cells, Cultured Dose-Response Relationship, Drug Humans Molecular Sequence Data Peptide Fragments Pigment Epithelium of Eye / cytology, drug effects, metabolism* Plasminogen Activators / biosynthesis* RNA, Messenger / metabolism Receptors, Cell Surface / biosynthesis* Receptors, Thrombin / chemistry, metabolism* Receptors, Urokinase Plasminogen Activator Recombinant Proteins Thrombin / pharmacology* Type C Phospholipases / pharmacology Urokinase-Type Plasminogen Activator / biosynthesis |
| Chemical | |
Reg. No./Substance:
|
0/PLAUR protein, human; 0/Peptide Fragments; 0/RNA, Messenger; 0/Receptors, Cell Surface; 0/Receptors, Thrombin; 0/Receptors, Urokinase Plasminogen Activator; 0/Recombinant Proteins; EC 3.1.4.-/Type C Phospholipases; EC 3.4.21.-/Plasminogen Activators; EC 3.4.21.5/Thrombin; EC 3.4.21.73/Urokinase-Type Plasminogen Activator |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Lymphocyte adhesive interactions with lacrimal gland acinar epithelial cells in primary culture.
Next Document: Cytokine modulation of adhesion molecule expression on human retinal pigment epithelial cells.